Electrospray-Differential Mobility Analysis System for the Analysis of Biomacromolecules, Polymeric (Bio)Materials and Nanoparticles

为了有效地开展他们在胶体和聚合物科学、软物质、微流体和再生医学方面的研究项目，蒙特利尔理工大学的一组研究人员迫切需要一种分析工具，可以快速表征非均质复杂纳米级系统（生物分子、聚合物和纳米颗粒）的大小和浓度。本提案的申请人已经确定了一种快速准确表征此类系统的分析方法，即电喷雾差分迁移率分析（ES-DMA）。本提案中要求的ES-DMA仪器能够在几分钟内分别确定分子量为~ 8kda至bbb100mda和分子量为2.5至1000nm的大分子和纳米颗粒的数量、浓度和大小分布。ES-DMA方法是最近开发的，目前还没有提供给魁北克的学术界和工业界。如果申请者的团队成员和当地科学界能够完全、畅通无阻地使用ES-DMA，将大大加快他们的研究项目的进展，克服非均质纳米系统所带来的尺寸表征挑战。

To effectively carry out their research projects in colloidal and polymer science, soft matter, microfluidics, and regenerative medicine, a group of researchers from Ecole Polytechnique de Montreal urgently require an analytical tool that can rapidly characterize heterogeneous complex nanosized systems (biomolecules, polymers and nanoparticles) for their the size and concentration. The applicants of this proposal have identified an analytical method for rapid and precise characterization of such systems, namely Electrospray-Differential Mobility Analysis (ES-DMA). The ES-DMA instrument requested in this proposal is able to determine number concentration and size distribution of macromolecules and nanoparticles with molecular weight and size ranging from ~8 kDa to > 100 MDa and from 2.5 to 1000 nm, respectively, within a few minutes. The ES-DMA method has been recently developed and is not currently available to the academic and industrial community in Quebec. A complete and unhindered access of the applicants' group members and the local scientific community at large to ES-DMA will greatly accelerate progress in their research programs by overcoming the size characterization challenge posed by heterogeneous nanosized systems.