Characterization of biological samples using Raman spectroscopy

未知样品的无损检测和分析在环境检测、工业检测、制药检测、食品和农业加工以及生物检测等领域产生了越来越多的机会。 拉曼光谱（RS）是无损检测中发展最快的光谱技术之一。RS利用光与分子的相互作用提供了在询问下的样品的化学指纹。拉曼光谱质量高度依赖于采集方法和仪器。 当处理生物介质时，它变得非常具有挑战性，因为生物分子可能表现出生动的多态性，并且在某些情况下，突变。 龙卷风光谱系统（TSS）建立光谱仪的基础上，专利发明称为高通量虚拟狭缝（HTVS）。改进的吞吐量使得可以在低采样体积和快速信号采集的条件下使用这种光谱仪，这通常是生物测试所需的情况。这种增强的性能需要更新的光谱库和分类协议，以充分发挥生物测试的潜力。这一挑战，提出了龙卷风光谱系统，将在此期间的合作进行调查。

Nondestructive testing and analysis of unknown samples has generated growing opportunities in fields such as environmental testing, industrial testing, pharmaceutical testing, food and agricultural processing as well as biological testing. Raman spectroscopy (RS) is one of the fastest growing spectroscopic techniques for nondestructive testing. RS provides a chemical fingerprint of a sample under interrogation utilizing the interaction of light with molecules. Raman spectral quality is highly dependent on the method of acquisition and instrumentation. It becomes highly challenging when dealing with biological media as biomolecules may exhibit vivid polymorphisms and, in certain cases, mutations. Tornado Spectral Systems (TSS) builds spectrometers based on a patented invention known as a high- throughput virtual slit (HTVS). The improved throughput makes it possible to use such a spectrometer in conditions of low sampling volumes and fast signal acquisition, which is generally the required case for biological testing. This enhanced performance requires updated spectral libraries and classification protocols to reach full potential for biological testing. This challenge, presented by Tornado-spectral systems, will be investigated during the period of this collaboration.