Fast Time-Resolved Fluorometer

这份提案是购买快速时间分辨荧光计的请求。荧光描述了染料通过发射光子来释放多余能量的过程。这个过程发生在几十纳秒的时间尺度上，时间分辨荧光仪(TRF)测量染料发出的荧光随着时间的推移消散的速度。TRF用于研究各种分子，这些分子用于塑料、洗涤剂、蛋白质、DNA和许多其他合成和天然产品。许多荧光研究探测一种染料与系统中引入的另一种分子之间的相互作用，以猝灭染料的荧光。猝灭发生的时间尺度提供了关于正在研究的系统的关键信息。例如，位于大分子疏水口袋中的染料被水溶性猝灭剂猝灭的速度很低，这表明疏水口袋内部的局部粘性很高，该大分子可以作为疏水药物缓释的载体。

This proposal is a request to purchase a fast time-resolved fluorometer. Fluorescence describes the process whereby a dye releases its excess energy by emitting a photon. This process occurs on a time scale of a few tens of nanoseconds and a time-resolved fluorometer (TRF) measures how quickly the fluorescence emitted by a dye dissipates over time. TRFs are used to study a variety of molecules which are used in plastics, detergents, proteins, DNA, and many other synthetic and natural products. Many fluorescence studies probe the interactions between a dye and another molecule that is introduced in the system to quench the fluorescence of the dye. The time scale over which quenching occurs provides critical information about the system under study. For instance, the low quenching rate of a dye located in the hydrophobic pocket of a macromolecule by a water-soluble quencher demonstrates that the interior of the hydrophobic pocket is highly viscous locally and that the macromolecule could be used as a delivery vehicle for the slow release of hydrophobic drugs.