X-ray Generator for Macromolecular Crystallography

我们需要理学MicroMax-003微焦点X射线发生系统，这将是SFU广泛的生物化学实验室的重要资源。 这种新仪器将取代SFU高分子衍射设施中不再起作用的双阳极X射线发生器。我们将使用该仪器：对抗生素和抗病毒靶点的蛋白质晶体进行衍射实验（Paetzel、克雷格、古特曼和洛温伯格实验室）;研究核酸和核酶的结构（Unrau实验室）;确定膜结合受体和酶的结构（Young和Cornell Labs）并解决与细胞凋亡和癌症相关的蛋白质复合物的结构（Pio Lab）。此外，SFU各部门的许多其他研究实验室将在经验丰富的晶体学家的监督和培训下使用该仪器（MBB，化学，生物学，物理学，生物医学生理学和运动学以及健康科学学院）。该X射线发生器的稳定性，耐用性和低维护将确保SFU的研究人员定期访问X射线束时间，用于衍射数据集收集以及测试其大分子晶体，以在同步加速器设施中进一步收集高分辨率数据。这种新仪器将是一个很大的好处，在SFU实验室，并将发挥重要作用，在新的抗生素，抗病毒药物，药物和治疗先天性心脏病，癌症和许多其他疾病的发展，以及促进真实的时间生物跟踪方法和纳米技术设备的发展。我们的研究领域和整个加拿大都将受益于获得这一仪器，并将大大有助于详细了解大量医学和科学上重要的生物大分子的结构。

We request a Rigaku MicroMax-003 Microfocus X-ray generation system that will be a great resource to a broad range of biochemistry laboratories at SFU. This new instrument will replace the no-longer functional rotating-anode X-ray generator that resides in the SFU Macromolecular Diffraction Facility. We will be using this instrument to: perform diffraction experiments on protein crystals of antibiotic and antiviral targets (Paetzel, Craig, Guttman, and Lowenberger Labs); investigate the structure of nucleic acids and ribozymes (Unrau Lab); determine the structure of membrane bound receptors and enzymes (Young and Cornell Labs) and solve the structures of protein complexes involved in apoptosis and cancer (Pio Lab). In addition, many other research labs in various Departments at SFU will have access to the instrument (MBB, Chemistry, Biology, Physics, Biomedical Physiology and Kinesiology and the Faculty of Health Sciences) under the supervision and training of experienced crystallographers. The stability, robustness and low-maintenance of this X-ray generator will assure that investigators at SFU have regular access to X-ray beam time for diffraction data set collection as well as for testing their macromolecular crystals for further high-resolution data collection at synchrotron facilities. This new instrument will be a great benefit to laboratories at SFU and will play an important role in the development of new antibiotics, antiviral drugs, medications and treatments for congenital heart disease, cancer and many other diseases, as well as facilitating the development of real time biological tracking methodologies and nanotechnology devices. Our research fields, and Canada in general, will benefit from the acquisition of this instrument and will contribute greatly to the detailed understanding of the structure of a large number of medically and scientifically important biological macromolecules.