3D Printing and Biofabrication Instrument

我们建议购买一种独特的3D生物打印仪器，该仪器将使与该提案相关的12名研究人员以及来自多伦多大学及其相关教学医院、约克大学和瑞尔森大学的其他研究人员能够基于人体细胞和各种细胞外基质材料（“生物墨水”）常规生物打印组织模型。该仪器将独特地补充我们团队在以下方面的专业知识：（a）下一代微制造生物打印头和生物打印机墨盒的开发，（B）生物墨水的配方及其凝胶化行为的表征，（c）生物打印软材料和组织的生物材料降解行为和生物力学特性，以及（d）组织模型的体外表征。我们的研究目标是系统验证生物打印软材料，这些材料包含多个共定位的细胞群，并概括了与完整组织相关的重要功能特征。

We propose the purchase of a unique 3D Bioprinting Instrument that will enable the twelve investigators associated with this proposal as well as other researchers from the University of Toronto and its associated teaching hospitals, from York University and from Ryerson University to routinely bio-print tissue models based on human cells and a variety of extracellular matrix materials ("bio-inks"). The instrument will uniquely complement our team's expertise related to (a) the development of next generation of microfabricated bio-printheads and bioprinter cartridges, (b) the formulation of bio-inks and characterization of their gelation behavior, (c) the biomaterial degradation behavior and biomechanical properties of bioprinted soft materials and tissues, and (d) the in vitro characterization of tissue models. Our research goal is the systematic validation of bio-printed soft materials that contain multiple co-localized cell populations and recapitulate important functional characteristics associated with intact tissues.