Novel monomer and polymer synthesis with antimicrobial function and their reaction with enzymes

获得性细菌感染影响了近5%的住院患者，并通过医疗设备传播。细菌有能力附着在用于制造这种装置的生物材料上，并促进生物膜的形成。这是一个持续的挑战，因为传统的抗生素系统治疗往往不足以克服细菌生物膜的复杂组织。随着加拿大和其他国家人口老龄化的加剧，需要具有益生菌和/或抗微生物功能的聚合物生物材料，以及在某些情况下的愈合特性，以更好地应对微生物挑战。这项资助的假设是，通过合成途径产生的含抗菌单体和耐细菌单体及其聚合物将产生功能性生物材料。有三个主要目标：1)研究先前在实验室中生成的新型氟化聚氨酯单体对唾液衍生酶的抗性，并使用这些单体生产具有优化物理性能的聚合物，以及对酶/细菌相互作用的抗性。2)将化学融入到新型抗菌单体中，从而产生具有实际物理性能和抵抗细菌生物膜形成的聚合物。3)利用合成的可降解水凝胶材料，促进宿主成纤维细胞的结缔组织生长，促进宿主内皮细胞的新血管生长，生成新的牙周组织来修复牙齿周围组织。上述所有3个目标的研究结果将结合起来，产生创新的新化学物质，并更好地了解单体化学结构对生物材料的物理、生化和生物学结果的影响，设想用于含有细菌的环境，为实现与加拿大医疗保健行业相关的产品取得成功的临床/商业影响带来重大挑战。这项研究正在培养生物医学工程领域的HQP（一个就业增长率为72%的领域——美国劳工统计局）。

Acquired bacterial infections affect nearly 5% of all hospitalized patients and are transmitted via medical devices. Bacteria have the ability to adhere to biomaterials used to fabricate such devices and promote the formation of biofilms. This is an on-going challenge as traditional systematic treatment by antibiotics is often insufficient to overcome the complex organization of the bacterial biofilms. With an increasing aging population in Canada and other countries, the need for polymeric biomaterials with pro-biotic and/or microbial resistant function along with healing character in some instances, is needed to better address microbial challenges. The hypothesis of this grant is that antimicrobial containing monomers and bacterial resistant monomers, and their polymers, generated from synthetic pathways will yield functional biomaterials. There are three principal objectives: 1) To study the resistance of novel urethane-fluorinated monomers generated previously in the laboratory to saliva derived enzymes, and to produce polymers with these monomers, that are optimized for physical performance properties, and resistance to enzyme/bacterial interactions. 2) To incorporate chemistry into novel antimicrobial monomers that will yield polymers with practical physical properties and resistance to bacterial biofilm formation. 3) To generate new periodontal tissues to repair tissue around teeth using a synthetic degradable hydrogel material which facilitates the growth of connective tissue from the host fibroblast cells, and new blood vessels from the host endothelial cells. The findings from all 3 of the above objectives will combine to yield innovative new chemistries and a greater understanding of the influence of monomer chemical structure on the physical, biochemical and biological outcomes of biomaterials, conceived for use where bacteria containing environments yield significant challenges towards achieving a successful clinical/commercial impact for products associated with Canada's healthcare industry. This research is training HQP in the biomedical engineering field (a field with 72% job growth rate- US Bureau of labor statistics).