Transposon-mediated gene exploration in barley and other cereals

突变等位基因的特征为正常生理功能的遗传学提供了有价值的见解。通过T-DNA插入、RNA干扰和转座子标记的诱变使研究人员能够更容易地研究特定基因及其功能。转座子介导的插入突变比其他确定基因功能的方法具有优势，特别是在遗传转化不常规的情况下。通过几种基因标记策略，包括Ac/ ds介导的插入突变，确定了参与发育的重要基因。在转座子运动过程中，转座子插入到一个新的染色体位置，可能导致基因正常功能的中断。一些外显等位基因发生参数化，这是等位基因之间的相互作用，导致一个等位基因的遗传表达变化。转座子也作为表观遗传现象的组成部分，无论是在单基因水平上还是在更大的染色体区域上。正在进行研究以了解这些沉默机制。

Characterization of mutant alleles provides valuable insights into the genetics of normal physiological functions. Mutagenesis via T-DNA insertion, RNA interference and transposon tagging enable researchers to more readily study specific genes and their functions. Transposon -mediated insertional mutagenesis has advantages over other approaches for determining gene function especially where genetic transformation is not routine. Important genes involved in development were identified using several gene-tagging strategies involving Ac/Ds-mediated insertional mutagenesis. During the transposon movement, the transposon inserts into a new chromosomal location and may cause interruption in gene’s normal function. Some epialleles undergo paramutation which is an interaction between alleles and leads to a heritable expression change of one allele. Transposons have also served as building blocks for epigenetic phenomena, both at the level of single genes and across larger chromosomal regions. Studies are being conducted for the understanding these silencing mechanisms.