Multimode Microplate Reader for Investigation of Biofilms and Design of Biosensing Assays

最近在理解细胞行为的复杂分子机制方面的进展导致了新技术的发展，这些技术提高了我们生活的整体质量。通过研究决定单一生物现象的多个方面，这已经成为可能。引进最先进的细胞成像多模式酶标仪将使圭尔夫大学的八名研究人员能够加速他们的研究，促进改变生活的生物医学技术的发展，以提高我们的健康和食品安全。该仪器将位于圭尔夫大学工程学院的生物纳米实验室并由其管理，在那里，它将对支持致病细菌生物膜的研究和生物传感测定的发展至关重要。这一不可或缺的工具将以最大能力运行多年，支持几个生物工程研究人员的研究计划，以及安大略兽医学院，安大略农业学院和生物科学学院3个系的教职员工。所要求的多模式酶标仪不仅将取代BioNano实验室目前使用了10年的设备，而且还将增加额外的功能（即，自动数字显微镜、荧光强度收集、发光、荧光偏振、时间分辨荧光、生物发光共振能量转移- BRET、TR-荧光共振能量转移- TR-FRET）来分析生理条件下的细胞，同时提供仅用单个设备而不是购买三种不同仪器进行各种测试的可能性。为学生和技术人员提供的培训计划将通过显著加强研究生研究和教育以及吸引新的合作来最大限度地提高投资回报。

Recent progress in understanding the complex molecular mechanisms of cells behavior has led to the development of novel technologies that have improved the overall quality of our life. This has been made possible by studying multiple aspects that determine a single biological phenomenon. The introduction of a state-of-the-art cell imaging multimode microplate reader will allow eight researchers of the University of Guelph to accelerate their studies and facilitate the development of life-changing biomedical technologies to enhance our health and the safety of our food. This instrument will be located in and managed by the University of Guelph's BioNano Laboratory of the School of Engineering, where it will be critical to support the investigation of pathogenic bacterial biofilms and development of biosensing assays. This indispensable tool will operate at maximum capacity for many years, supporting the research programs of several biological engineering researchers and also faculty members across 3 departments of the Ontario Veterinary College, Ontario Agricultural College and the College of Biological Science. The requested multimode microplate reader will not only replace the 10 year old equipment currently in the BioNano Laboratory, but will also add extra capabilities (i.e., automated digital microscopy, collection of fluorescence intensity, luminescence, fluorescence polarization, time-resolved fluorescence, bio-luminescence resonance energy transfer - BRET, TR-Fluorescence Resonance Energy Transfer - TR-FRET) to analyse cells under physiological conditions while offering the possibility to perform a variety of tests with only a single equipment instead of purchasing three different instruments. Training programs offered to students and technical personnel will maximize the return of this investment by significantly enhancing graduate research and education, and also by attracting new collaborations.