"Sensing, transfer and regulation of ions and gases: Studies in a model organism, the zebrafish (Danio rerio)"

本研究计划侧重于对鱼类适应波动环境具有根本重要性的两个关键方面；调节它们体液的离子组成以及呼吸气体O2和CO2的感知和转移。虽然我们已经了解了很多关于离子在鳃和皮肤等上皮细胞上运动的机制，但实际上我们对离子调节的代谢成本一无所知——在细胞中移动离子需要消耗多少（能量）？我将用斑马鱼幼虫表皮离子运输细胞的离子摄取作为模型系统来研究这个问题。我的学员和我将使用最先进的扫描极谱和罐装离子选择电极技术来同时测量离子和O2进入离子细胞的通量，以提供离子调节代谢成本的第一个直接估计。离子调节领域的进一步研究将集中于建立调节机制，使鱼类在环境挑战（如环境氧气降低（缺氧）和盐度变化）中调节离子摄取速率。

This research proposal focuses on two key aspects of fundamental importance to the adaptation of fish to fluctuating environments; regulating the ionic composition of their body fluids and the sensing and transfer of the respiratory gases O2 and CO2. While we have learned much about the mechanisms of ion movement across epithelial such as gills and skin, virtually nothing is know about the metabolic cost of ion regulation - what does it cost (in energy) to move ions across a cell? I will examine this question using ionic uptake by the epidermal ion-transporting cells of zebrafish larvae as a model system. My trainees and I will use state-of-the-art scanning polarographic- and canning ion-selective electrode techniques to simultaneously measure ion and O2 fluxes into ionocytes to provide the first direct estimates of the metabolic cost of ion regulation. Additional research in the field of ion regulation will concentrate on establishing the regulatory mechanisms allowing fish to adjust ion uptake rates during environmental challenges such as lowered ambient O2 (hypoxia) and changing salinity.