Optimization of sampling, detection and characterization of subsurface microbial life in systems with low permeability and low biomass-sedimentary and crystalline settings

在证明地质隔离的深部处置库（深部地质处置库：DGR）的安全性方面，一个关键的组成部分是证明地质系统中的微生物不会对处置库的完整性以及当地环境（包括地下水和地表生态系统）造成影响。最近的研究表明，微生物生活在地球岩石圈的程度远远超过以前的想象。虽然这些生物体可能以非常低的丰度和/或无柄或孢子化状态原位存在，但随着时间的推移，条件的变化可能会刺激原位微生物活性。为了在加拿大DGR的规划和建设过程中预测和管理这种影响的可能性，有必要开发能够以最大的灵敏度检测和识别地质系统内微生物的方法。

A key component in demonstrating the safety of a deep geologically isolated repositories (Deep Geologic Repositories: DGR) is the demonstration that microorganisms indigenous to the geologic system do not pose a concern to the integrity of the repository and thus to the local environment, including groundwater and surface-based ecosystems. Recent research demonstrates that microbial life inhabits the Earth's lithosphere to a far greater extent than was previously thought. While these organisms may be present in situ at very low abundances and/or in sessile or sporulated states, changing conditions over time could result in stimulation of in situ microbial activity. In order to predict and manage the potential for such impacts during planning and construction of a Canadian DGR, it is necessary to develop methods that can detect and identify microorganisms living within geologic systems with the greatest possible sensitivity.