Enhanced bioremediation of nutrient contaminated groundwater

在马尼托巴，约25%的浅农村生活用水威尔斯井中含有超过饮用水标准的硝酸盐（Frost 2006）。补救技术可以解决散装肥料和养分储存设施下的养分污染土壤和地下水，这对农村地区的健康饮用水至关重要。其中一种技术，即强化生物修复，包括添加碳源、电子受体和/或大量或微量营养素，以促进原生微生物种群对硝酸盐的降解（ITRC 2000）。强化生物修复的好处包括促进自然微生物种群、限制对土地的扰动以及可持续的绿色修复解决方案。马尼托巴大学和Amec Foster惠勒之间的这项研究合作考察了乙醇作为提高硝酸盐反硝化能力的碳源。乙醇在较冷气候下具有较高的脱氮率，土壤柱显示在数周内脱氮率降低95%（Martin等人，2009年; Edwards等人，2007年）。作为这项研究的一部分，来自营养污染的肥料储存设施的土壤和地下水将被用来创建微观世界，以评估寒冷气候条件下的硝酸盐去除率。将在不同处理之间评价微生物种群，以确定乙醇注射对微生物生长/代谢的影响。实验室土柱测试将检查乙醇的运动，以了解和预测通过含水层的运动。这些结果将形成一个数值流模型的基础上，该网站为未来实施的现场规模的补救处理计划。这项研究是至关重要的，以了解微生物和含水层材料的乙醇添加反应，并帮助开发具有成本效益和及时的解决方案，硝酸盐修复。

In Manitoba approximately 25% of shallow rural domestic water wells contained nitrate exceeding the drinking water standards (Frost 2006). Remediation techniques that can address nutrient contaminated soil and groundwater beneath bulk fertilizer and nutrient storage facilities are crucial for healthy drinking water in rural areas. One such technique, enhanced bioremediation, includes the addition of a carbon source, electron acceptors, and/or macro or micro nutrients to promote nitrate degradation by native microbial populations (ITRC 2000). The benefit of enhanced bioremediation includes the promotion of the natural microbial population, limited disturbance of the land, and a sustainable green solution to remediation. This research collaboration between the University of Manitoba and Amec Foster Wheeler examines ethanol as a carbon source for improving nitrate denitrification capacity. Ethanol has shown promise for high denitrification rates in colder climates, with soil columns showing 95% reduction within weeks (Martin et al. 2009; Edwards et al. 2007). As part of this research, soil and groundwater from a nutrient contaminated fertilizer storage facility will be used to create microcosms to evaluate the nitrate removal rates under cold climate conditions. Microbial populations will be evaluated between the different treatments to determine the effect of the ethanol injections on microbial growth/metabolism. A laboratory soil column test will examine the movement of ethanol to understand and predict the movement through the aquifer. These results will form the basis for a numerical flow model of the site for the future implementation of a field-scale remediation treatment plan. This research is critical to understand the microbial and aquifer material response to ethanol addition and help develop cost effective and timely solution for nitrate remediation.