E-cadherin Mechanotransduction, Pluripotency and the Warburg Effect

胚胎干细胞(ESCs)是多能的，这意味着它们有能力分化为体内的任何细胞类型。我们观察到，当mESC聚集体在生物反应器中搅拌时，小鼠(M)ESCs的多能性得到促进。即使去除了维护因子LIF，也能保持多能性。此外，细胞重编程，即细胞去分化为多能性的过程，在生物反应器中发生的效率要高出1000倍。我们的初步数据表明，在搅拌引起的流体剪切作用下，多能转录因子β-连环蛋白被移位到细胞核中。

Embryonic stem cells (ESCs) are pluripotent meaning they have the ability to differentiate into any cell type in the body. We have observed that the pluripotency of murine (m)ESCs is promoted when mESC aggregates are stirred in bioreactors. Pluripotency is maintained even when the maintenance factor LIF is removed. Moreover, cellular reprogramming, the process of dedifferentiating cells to pluripotency, occurs 1000-fold more efficiently in the bioreactor. Our preliminary data indicates that the pluripotency transcription factor β-catenin is translocated into the nucleus in response to fluid shear caused by stirring.