Single-molecule visualization and manipulation of protein and DNA interactions and dynamics, on nanoscale dimensions

我的研究计划的首要目标是可视化、定量分析和了解蛋白质-DNA和蛋白质-蛋白质相互作用，它们在调节细胞过程中发挥着关键的生物物理作用。我对蛋白质和DNA分子等生物聚合物的动态、结构和遗传特性之间的相互作用很感兴趣，可视化和纳米操纵工具的进步，以及对受限分子的理论处理，使我们能够更详细地了解这些特性。这项发现拨款将使我的团队能够利用我们的单分子显微镜工具来探索关于蛋白质和DNA系统在纳米尺度上的行为的公开问题；这些问题是其他方法无法获得的，具有国际研究兴趣和影响。

The overarching objective of my research program is to visualize, quantitatively analyze and understand protein-DNA and protein-protein interactions, which play key biophysical roles in regulating cellular processes. I am fascinated by the interplay between the dynamic, structural, and genetic properties of biopolymers such as protein and DNA molecules, which advancements in visualization and nano manipulation tools, as well as theoretical treatments of confined molecules, are allowing us to understand in further detail. This Discovery Grant will enable my group to leverage our single-molecule microscopy tools to explore open questions about the behavior of protein and DNA systems on nanoscale dimensions; questions which are inaccessible to other methods, and which have international research interest and impact.