Investigating optimal surface coating strtegies for linking SQI scapture probes on plastic amd glass subtrates

该项目的目的是提供一种具有成本效益的策略，将蛋白质粘附到玻璃和塑料底物（SQI Diagnostics目前使用的主要底物）上，这些底物稳定，功能齐全，并与底物正确结合。该项目将有三个主要步骤来实现这一目标：(A)表面功能化，(B)蛋白质固定化和(C)生物功能评估。我们建议研究生物分子表面稳定和共价附着的几种功能化学。我们还提出了几种将生物分子固定在表面上的方法，如传统的液相涂层以及用于固定SQI所需生物分子的自动打印。作为对照，我们还将研究表面上没有共价附着的生物分子的物理模式，以及使用该公司目前使用的相同功能表面。为了证明生物功能，涂层将使用SQI目前可用的生物测定方法进行优化。

The aim of this project is to offer a cost-effective strategy for attaching proteins to glass and plastic substrates (the main substratesthat SQI Diagnostics currently uses) that are stable, functional and properly bond to the substrates. This project will have threemain steps toward reaching this goal: (A) Surface functionalization, (B) Protein immobilization and (C) Bio-functionalityassessment.We propose to investigate several functional chemistries on the surfaces for stable and covalent attachment of biomolecules. Wealso propose several methods for the immobilization of biomolecules onto the surfaces such as conventional liquid phase coatingas well as automated printings for immobilizing SQI's desired biomolecules. As controls, we will also investigate physicalpatterning of the biomolecules on the surface without covalent attachment as well as using the same functional surfaces that thecompany is currently using. To demonstrate the bio-functionality, the coatings will be optimized using the currently availablebioassays at SQI.