Developing novel smart biointerfaces for in vitro biosensing and point of care diagnostics

开发用于体外生物传感和护理诊断的新型智能生物接口

• 批准号: 537197-2018
• 负责人: Didar, Tohid
• 金额: $ 14.57万
• 依托单位: McMaster University
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=699345
• 关键词: Developing; novel; smart; biointerfaces; vitro

In this project, we propose to design a new class of immunodiagnostic-based biosensors that are more precise and functional than conventional biosensors. We will do this using our novel technique in preparation of the sensors' bio-interfaces where we utilize the lubricant-infused-surface technology in order to enhance the sensor's functionality. Our new surface modification techniques allow us to obtain surfaces that have a superior ability in detecting very low concentrations of the desired bio-species in complex environments such as blood and blood plasma. We will employ these biosensors in detecting low-molecular weight proteins such as Interleukin 6 which plays a crucial role in early detection of a variety of cancers as well as inflammatory diseases and immunotherapy. The other objective of this project is producing point-of-care diagnostic biosensors by incorporating microfluidic systems. These portable devices can facilitate the detection of the related diseases in a rapid and straightforward, yet precise manner. Creation of functional antibodies referred to as "biofunctional inks" is another goal of this proposal. This product can target a wide range of biosensor industries and biomedical applications, where sensor's robustness, ease of fabrication, and versatility are desired. We intend to commercialize our final products in collaboration with our industry partner, SQI Diagnostics Inc. We will investigate the long-term stability of the developed biosensors and determine the proper storage conditions of the products. Further, we will design high throughput production lines with in-line quality control systems for the fabrication of the biosensors and other side products.

在这个项目中，我们建议设计一种新的基于免疫诊断的生物传感器，它比传统的生物传感器更精确和更具功能性。我们将使用我们的新技术来准备传感器的生物接口，其中我们使用注入润滑剂的表面技术来增强传感器的功能。我们的新表面改性技术使我们能够获得在复杂环境(如血液和血浆)中检测所需生物物种的极低浓度的卓越能力的表面。我们将使用这些生物传感器来检测低分子蛋白质，如白介素6，它在多种癌症的早期检测以及炎症性疾病和免疫治疗中起着至关重要的作用。该项目的另一个目标是通过结合微流控系统来生产护理点诊断生物传感器。这些便携设备可以帮助以快速、直接但准确的方式检测相关疾病。创造被称为“生物功能墨水”的功能性抗体是这项提议的另一个目标。该产品可以面向广泛的生物传感器行业和生物医学应用，在这些应用中，传感器的坚固性、易于制造和多功能性是人们所期望的。 我们打算与我们的行业合作伙伴SQI诊断公司合作，将我们的最终产品商业化。我们将调查开发的生物传感器的长期稳定性，并确定产品的适当储存条件。此外，我们将设计具有在线质量控制系统的高通量生产线，用于制造生物传感器和其他副产品。