Developing novel smart biointerfaces for in vitro biosensing and point of care diagnostics

在这个项目中，我们建议设计一类新的免疫诊断为基础的生物传感器，比传统的生物传感器更精确和功能。我们将使用我们的新技术来制备传感器的生物界面，其中我们利用润滑剂注入表面技术来增强传感器的功能。我们新的表面改性技术使我们能够获得具有上级能力的表面，在复杂环境（如血液和血浆）中检测非常低浓度的所需生物物种。我们将利用这些生物传感器检测低分子量蛋白质，如白细胞介素6，它在各种癌症的早期检测以及炎症性疾病和免疫治疗中起着至关重要的作用。该项目的另一个目标是通过结合微流体系统生产即时诊断生物传感器。这些便携式设备可以方便地以快速和直接而精确的方式检测相关疾病。被称为“生物功能油墨”的功能抗体的产生是本提议的另一个目标。该产品适用于各种生物传感器行业和生物医学应用，这些行业需要传感器的坚固性、易于制造性和多功能性。我们打算与我们的行业合作伙伴SQI Diagnostics Inc.合作，将我们的最终产品商业化。我们将调查开发的生物传感器的长期稳定性，并确定产品的适当储存条件。此外，我们将设计高通量生产线，并配备在线质量控制系统，用于生物传感器和其他副产品的制造。

In this project, we propose to design a new class of immunodiagnostic-based biosensors that are more precise and functional than conventional biosensors. We will do this using our novel technique in preparation of the sensors' bio-interfaces where we utilize the lubricant-infused-surface technology in order to enhance the sensor's functionality. Our new surface modification techniques allow us to obtain surfaces that have a superior ability in detecting very low concentrations of the desired bio-species in complex environments such as blood and blood plasma. We will employ these biosensors in detecting low-molecular weight proteins such as Interleukin 6 which plays a crucial role in early detection of a variety of cancers as well as inflammatory diseases and immunotherapy. The other objective of this project is producing point-of-care diagnostic biosensors by incorporating microfluidic systems. These portable devices can facilitate the detection of the related diseases in a rapid and straightforward, yet precise manner. Creation of functional antibodies referred to as "biofunctional inks" is another goal of this proposal. This product can target a wide range of biosensor industries and biomedical applications, where sensor's robustness, ease of fabrication, and versatility are desired.We intend to commercialize our final products in collaboration with our industry partner, SQI Diagnostics Inc. We will investigate the long-term stability of the developed biosensors and determine the proper storage conditions of the products. Further, we will design high throughput production lines with in-line quality control systems for the fabrication of the biosensors and other side products.