Developing novel smart biointerfaces for in vitro biosensing and point of care diagnostics

在这个项目中，我们建议设计一种新的基于免疫诊断的生物传感器，它比传统的生物传感器更精确和功能更强。我们将使用我们的新技术来制备传感器的生物界面，我们利用润滑油注入表面技术来增强传感器的功能。我们的新表面修饰技术使我们能够获得在复杂环境（如血液和血浆）中检测极低浓度所需生物物种的卓越能力的表面。我们将使用这些生物传感器来检测低分子量蛋白质，如白细胞介素6，它在各种癌症的早期检测以及炎症疾病和免疫治疗中起着至关重要的作用。该项目的另一个目标是通过结合微流体系统生产即时诊断生物传感器。这些便携式设备可以快速、直接、精确地检测相关疾病。创造被称为“生物功能墨水”的功能性抗体是该提案的另一个目标。该产品可以针对广泛的生物传感器行业和生物医学应用，其中传感器的稳健性，易于制造和多功能性是必需的。我们打算与我们的行业合作伙伴SQI Diagnostics Inc.合作，将我们的最终产品商业化。我们将研究开发的生物传感器的长期稳定性，并确定产品的适当储存条件。此外，我们将设计具有在线质量控制系统的高通量生产线，用于制造生物传感器和其他副产品。

In this project, we propose to design a new class of immunodiagnostic-based biosensors that are more precise and functional than conventional biosensors. We will do this using our novel technique in preparation of the sensors' bio-interfaces where we utilize the lubricant-infused-surface technology in order to enhance the sensor's functionality. Our new surface modification techniques allow us to obtain surfaces that have a superior ability in detecting very low concentrations of the desired bio-species in complex environments such as blood and blood plasma. We will employ these biosensors in detecting low-molecular weight proteins such as Interleukin 6 which plays a crucial role in early detection of a variety of cancers as well as inflammatory diseases and immunotherapy. The other objective of this project is producing point-of-care diagnostic biosensors by incorporating microfluidic systems. These portable devices can facilitate the detection of the related diseases in a rapid and straightforward, yet precise manner. Creation of functional antibodies referred to as "biofunctional inks" is another goal of this proposal. This product can target a wide range of biosensor industries and biomedical applications, where sensor's robustness, ease of fabrication, and versatility are desired.We intend to commercialize our final products in collaboration with our industry partner, SQI Diagnostics Inc. We will investigate the long-term stability of the developed biosensors and determine the proper storage conditions of the products. Further, we will design high throughput production lines with in-line quality control systems for the fabrication of the biosensors and other side products.