Developing an Ig-Seq workflow for analyzing populations of rabbit monoclonal antibodies

该项目将启动威勒斯实验室之间的第一次合作，在威勒斯大学和免疫抗体有限公司位于维多利亚，不列颠哥伦比亚省免疫抗体有限公司是一家本地生物技术公司，在定制抗体业务超过25年的经验。他们的主要产品之一包括他们的专有技术，用于使用他们的技术生成定制的兔单克隆抗体，该技术筛选兔的免疫库，以直接从B细胞中选择所需的抗体。这个Engage项目的主要目标是开发一个工作流程，用于分析这些兔子系统中产生的抗体群体，使用下一代测序作为简化抗体生产过程的一种方式。主要挑战将是1）从免疫细胞群体获得测序数据和2）使用生物信息学分析所得序列数据。Willerth实验室将提供人员和专业知识来实现这些目标。该项目将培养生物技术、细胞培养和生物信息学领域的高素质人才。

This project will initiate a first time collaboration between the Willerth laboratory at the University of Victoriaand Immunoprecise Antibodies Ltd. located in Victoria, B.C. Immunoprecise Antibodies Ltd. is a localbiotechnology company with over 25 years of experience in the custom antibody business. One of their majorproducts includes their proprietary technology for generating custom rabbit monoclonal antibodies using theirtechnology, which screens the immune repertoire of the rabbit for selecting the desired antibody directly fromB cells. The major goal of this Engage project is to develop a work flow for analyzing the antibody populationsproduced in these rabbit systems using next generation sequencing as a way to streamline the antibodyproduction process. The major challenges will be 1) obtaining sequencing data from the population of immunecells and 2) analyzing the resulting sequence data using bioinformatics. The Willerth lab will provide personneland expertise to achieve these goals. This project will train highly qualified personnel in the areas ofbiotechnology, cell culture, and bioinformatics.