Impact of fungicide treatment on microbial communities associated with late season root rot in canola

拜耳作物科学为加拿大农民提供作物保护解决方案，专注于为加拿大油菜籽生产商提供管理选项，以保护油菜籽作物在种子萌发期间和整个生长季节免受病原体侵袭。大草原油菜籽生产商正面临晚季根腐病这一新出现的问题，这可能会给生产商带来巨大的产量和经济损失(CanolaWatch，2014)。在常规种子处理的有效时期之后，出现了晚季病理，使这些处理对晚季根腐病的防治无效。之前的一项Engage赠款使用了约斯特实验室的专业知识和基础设施来量化与遭受晚季根腐病症状的油菜籽植物相关的微生物群落。结果表明，钩端螺旋体菌科真菌与植物组织病理学关系最为密切，与Orchbactrum和Shingobacter群的关系最为密切。Engage项目产生的数据集为通过Engage Plus计划继续这项研究提供了必要的工具。目前的提案将研究拜耳作物科学公司正在开发的一种实验性杀菌剂对经过处理的油菜籽作物微生物群落结构的影响，这些作物生长在以前受Bylate季节根腐病影响的田地中。这些结果将提供微生物群落的高分辨率量化，并了解微生物群落成员如何受到实验化合物的影响。这些数据将使拜耳作物科学公司更好地了解实验杀菌剂在防止晚季根腐病方面的作用机理及其对土著土壤和内生微生物区系的影响。这一结果将直接有利于拜耳作物科学公司推进虫害管理和产品开发工作。

Bayer CropScience provides crop protection solutions to Canadian farmers, with a large commercial focus forproviding Canadian canola producers with management options to protect canola crops from pathogens, duringseed emergence and throughout the growing season. Prairie canola producers are encountering an emergingproblem of late season root rot, which can result in significant yield and economic losses to producers (CanolaWatch, 2014). The late season pathology appears after the efficacy period of conventional seed treatments,rendering these treatments ineffective in the control of late season root rot. A previous Engage grant used theexpertise and infrastructure of the Yost lab to quantify the microbial communities that are associated withcanola plants suffering from late season root rot symptoms. It was found that fungal organisms belonging to theLeptosphaeriacaea family were most closely associated with plant tissue pathology, and were notablyassociated with bacterial groups of Orchobactrum and Shingobacterium. The data-sets generated from theEngage project provides the necessary tools for continuing this research through the Engage Plus program. Thecurrent proposal will study the effects of an experimental fungicide, in development by Bayer CropScience, onthe microbial community structure of treated canola crops that are grown in field plots previously impacted bylate season root rot. The results will provide a high-resolution quantification of the microbial communities andan understanding of how the microbial community members are impacted by the experimental compound.This data will provide Bayer CropScience with a greater understanding of the mechanisms of action of theexperimental fungicide in preventing late season root rot and its impact on the indigenous soil and endophyticmicrobiota. The results will be of direct benefit to Bayer CropScience by advancing their pest managementproduct development efforts.