Cell Imaging Multi-Mode Reader

准确测量代谢和细胞信号过程的数量和活动对于了解体内的生理和病理过程至关重要。为了进行这些类型的分析，我们需要一个多模式阅读器。我们目前的多模式阅读器由于已经使用了20年，已经无法使用和不可修复，因此购买新的多模式阅读器成为当务之急。获得多模式阅读器对我们研究计划的成功至关重要，如果没有这种设备，我们的研究计划将受到极大的限制。收购Cytation 5细胞成像和多模式阅读器不仅将使我们能够进行这项重要的研究，而且它还将通过允许同时量化代谢和细胞信号通路，同时提供这些细胞过程的可视化，从而扩展我们目前的研究能力。这一点很重要，因为亚细胞位置和信号组件之间的相互作用提供了关于这些复杂且通常高度集成的信号通路的关键信息。使用所需的设备，我们将能够同时量化细胞信号组件的数量、亚细胞位置和相互作用，从而极大地增加我们研究计划输出的数据的丰富性。重要的是，Cytation 5的高质量组件使用比传统分析系统更具成本效益的简化方法，可以产生高度准确的结果。此外，Cytation 5系统附带的Gen5软件通过直观的分析平台提供高质量的图像和精确的数据。使用Cytation 5，我们将能够扩展我们对支撑组织(肌肉、肝脏和大脑)以及细胞(肌肉、肝脏、癌症)内基本生理过程的机制的理解，这将大大有助于我们理解肌肉、肝脏和大脑代谢功能的调节，以及细胞死亡和分化的调节。Cytation 5将支持滑铁卢大学运动学系5名申请者的研究项目。此外，购买这种设备将成为培训下一代加拿大科学家的关键基础设施。直接访问Cytation 5将使学员能够高效地完成他们的分析，并为他们提供成为学术和行业职业生涯中领先的研究人员所需的基础和新颖技能。总体而言，收购Cytation 5细胞成像和多模式阅读器将极大地提高我们和我们的学员正在进行的研究的数量和质量，并将提高我们作为加拿大领域研究领先者的地位。

Accurately measuring the quantity and activities of metabolic and cell signalling processes is crucial in understanding physiological and pathological processes within the body. In order to conduct these types of analyses we require a multi-mode reader. Our current multi-mode reader is non-functioning and non-repairable since it is 20 years old, making acquisition of a new multi-mode reader an urgent matter. Acquisition of a multi-mode reader is vital to the success of our research programs and without this equipment our research programs would be dramatically limited. Acquisition of the Cytation 5 Cell Imaging and Multi-Mode Reader will not only allow us to conduct this vital research, but it will expand our current research capacity by allowing simultaneous quantification of metabolic and cell signalling pathways while also providing visualization of these cellular processes. This is important because subcellular location and the interaction between signaling components provide critical information on these complex and often highly integrated signaling pathways. Using the requested equipment we will be able to simultaneously quantify quantity, subcellular location and interaction of cell signaling components, dramatically increasing the richness of the data put out by our research programs. Importantly, the high quality components of the Cytation 5 yield highly accurate results, using simplified methods that are more cost effective than traditional analytical systems. Furthermore, the Gen5 software that accompanies the Cytation 5 system yields high quality images and precise data with an intuitive analysis platform. Using the Cytation 5, we will be able to expand on our understanding of the mechanisms that underpin fundamental physiological processes within tissues (muscle, liver and brain) as well as cells (muscle, liver, cancer), which will contribute substantially to our understanding of the regulation of muscle, liver, and brain metabolic function, and the regulation of cell death and differentiation. The Cytation 5 will support the research programs of 5 applicants within the Department of Kinesiology at the University of Waterloo. Furthermore, acquisition of this equipment will serve as a critical piece of infrastructure used to train the next generation of Canadian scientists. Direct access to the Cytation 5 will allow trainees to complete their analyses efficiently and give them the foundational and novel skills required to become leading researchers in both academic and industry careers. Overall, the acquisition of the Cytation 5 Cell Imaging and Multi-Mode Reader will greatly enhance the quantity and quality of the research we and our trainees are conducting and will increase our standing as research leaders in our fields in Canada.