Development of a vaccine against avian reovirus infection in broiler chickens

禽呼肠孤病毒（ARV）是一种经济上重要的新出现的家禽疾病的病原体。关节源性ARV引起腱鞘炎/关节炎综合征，其特征在于跗关节的单侧或双侧 ** 肿胀，导致跛行。根据严重程度，抗逆转录病毒感染导致生长不良和产量下降，有时甚至死亡，给家禽业造成相当大的经济损失。虽然有商业疫苗，但新出现的抗逆转录病毒强毒株的发生率 ** 继续影响加拿大家禽业，这表明目前的疫苗对 ** 流行的抗逆转录病毒毒株无效。接种疫苗是保护肉鸡免受抗逆转录病毒感染的首选手段。目前，加拿大的一些农场使用自体疫苗预防鸡感染抗逆转录病毒病毒;然而，** 自体疫苗诱导的免疫力不可预测，作为抗逆转录病毒病毒的专用控制措施 ** 的潜力有限。没有有效的疫苗可以对付目前在 ** 加拿大肉鸡业流行的新出现的抗逆转录病毒株。如果抗逆转录病毒药物继续造成疾病，加拿大肉鸡 ** 业将继续遭受重大经济损失。因此，根据ARV流行强毒株的特征开发安全有效的疫苗至关重要。我们已经在遗传和免疫学水平上对萨斯喀彻温省肉鸡产业中出现的抗逆转录病毒变异株进行了鉴定。我们已经证明，四种基因型的抗逆转录病毒影响萨斯喀彻温省肉鸡 ** 鸡产业，这些基因型不能控制的抗逆转录病毒疫苗只含有一种类型。我们 ** 建议开发一种安全有效的抗ARV多价活疫苗和灭活疫苗，专门 ** 为加拿大家禽业量身定制，以控制新出现的ARV感染。

Avian reoviruses (ARVs) are etiological agents for an economically important emerging poultry disease.**Arthrogenic ARVs cause tenosynovitis/arthritis syndrome which is characterized by unilateral or bilateral**swelling of the hock joint resulting in lameness. Depending on the severity, ARV infection results in poor**growth and poor production, and sometimes death, causing considerable economic losses to the poultry**industry. While commercial vaccines are available, the incidence of emerging virulent strains of ARV that**continue to impact the Canadian poultry industry suggest that current vaccines are not effective against**circulating ARV strains. Vaccination is the preferred means to protect broiler chickens from ARV infection.**Currently, some farms in Canada use autogenous vaccines to prevent ARV infection in chickens; however,**autogenous vaccines induce unpredictable immunity and have limited potential as a dedicated control measure**against ARVs. There is no effective vaccine available against the emerging ARV strains currently circulating in**the Canadian broiler chicken industry. If ARVs continue to cause disease, the Canadian broiler chicken**industry will continue to suffer significant economic losses. Therefore, the development of a safe and an**effective vaccine based on the characteristics of circulating virulent strains of ARV is essential. We have**characterized emerging variant ARVs from the broiler chicken industry in Saskatchewan at the genetic and**immunological level. We have demonstrated that four genotypes of ARV affect the Saskatchewan broiler**chicken industry and these genotypes cannot be controlled by a ARV vaccine containing just one type. We**propose to develop a safe and effective multivalent live and inactivated vaccine against ARVs specifically**tailored for the Canadian poultry industry to control emerging ARV infections.