Development of potential alternative fluorophores to Sybr Green to stain double stranded DNA******

荧光染料在与双链DNA结合时发光，在定量聚合酶链反应（qPCR）和高分辨率熔融（HRM）实验等应用中至关重要，这两种技术都是分子生物学中的重要技术。这些技术在生命科学、法医学、人类健康等领域得到了广泛的应用。虽然目前使用的染料在不同程度上满足qPCR和HRM的要求，但许多染料价格昂贵或效率不够高。本项目将研究基于1,8-萘酰亚胺核心结构的替代染料。1,8-萘酰亚胺荧光染料通常很容易合成，一些类似物具有独特的荧光性质，适合于qPCR和HRM应用。本研究将产生1,8-萘酰亚胺染料，这些染料很容易获得，并且可能适用于qPCR和HRM应用。

Fluorescent dyes that emit light upon binding to double stranded DNA are crucial in applications such as quantitative Polymerase Chain Reactions (qPCR) and High Resolution Melt (HRM) experiments, both important techniques in molecular biology. These techniques have found a wide range of applications in life science, forensics, human health and beyond. While the dyes that are currently in use fulfill the requirements for qPCR and HRM to various degrees, many are expensive or not efficient enough for the applications. This project will investigate alternative dyes based on 1,8-naphthalimide core structures. The 1,8-naphthalimide fluorescent dyes are usually straightforward to synthesize, and some analogs have unique fluorescent properties that are suitable for qPCR and HRM applications. This research will generate 1,8-naphthalimide dyes that are readily available, and potentially suitable for both qPCR and HRM applications.