Real time RT qPCR equipment ( QS5 QPCR System , Thermo Fisher Scientific)

作为一个合作研究团队，我们正在要求Q5S qPCR系统(定量聚合酶链式反应)来加强我们的联邦资助的研究计划，旨在改善药物开发、配方、给药和治疗效果。我们实验室的现有设备已有20多年的历史，由于持续的技术问题以及与用于基因表达研究的现代试剂混合物的不兼容，需要更换。至关重要的是，我们必须用一台新的qPCR机取代我们目前的qPCR机，因为这台仪器对于我们合作中做得不够的实验是必不可少的。Q5S定量聚合酶链式反应系统与不同供应商销售的其他型号相比是通用的，符合所有参与我们项目的研究人员的要求。我们的合作研究项目，包括Mishra博士、Hoare博士、Selvan anapathy博士、Gupta博士和Niles博士，由NSERC、CIHR、CHRP、Grants和NSERC的Discovery Grants计划资助。Hoare博士和Selvan anapathy博士是其他RTI补助金的共同申请者，与这里要求的设备无关；因此，他们不能列在本申请中。我们由代表化学工程系、机械工程系、生物学系、精神病学系和行为神经科学系的教职员工组成。我们的研究项目包括药物开发、配方和给药，包括针对中枢神经系统疾病的鼻腔给药纳米技术平台。实时定量聚合酶链式反应仪器将用于量化在疾病状态下改变并受我们开发的新药和药物配方调控的基因的基因表达。测量基因表达的实验将在各种脑部疾病的细胞、蠕虫和临床前啮齿动物模型中进行。麦克马斯特附近的实验室没有类似的设备；因此，采购这种仪器对于我们进行实验和推进我们的联邦资助的合作研究项目至关重要。该仪器的问世将极大地推进我们的创新研究计划，并促进旨在开发新药物化合物和改善药物向大脑输送的实验合作。这最终将导致治疗效果的提高，旨在达到治疗神经退行性疾病的临床阶段。此外，我们的研究将有助于该领域对新型神经营养因子及其分子机制的理解。购买此仪器将立即使我们的所有学生受益，因为他们可以更方便地使用当前行业标准的机器，并获得更多体验。最终，从我们的研究项目中获得的结果将使加拿大和国际社会在知识转化和传播治疗脑部疾病的新疗法方面受益匪浅。

As a collaborative research team, we are requesting the Q5S qPCR system (quantitative- polymerase chain reaction) to enhance our federally funded research programs aimed at improving drug development, formulations, delivery and therapeutic efficacy. The current equipment in our laboratory is more than 20 years old and needs replacing due to on-going technical issues and incompatibilities with modern reagent mixtures for gene expression studies. It is critical that we replace our current qPCR machine for a new one as this instrument is essential to the experiments underdone in our collaborations. The Q5S qPCR system is versatile as compared to other models marketed by different suppliers and meets the requirements of all the researchers involved in our projects. Our collaborative research programs, which include Drs. Mishra, Hoare, Selvaganapathy, Gupta and Niles, are funded by NSERC, CIHR, CHRP, grants and NSERC's Discovery Grants program. Drs. Hoare and Selvaganapathy are co--applicants on other RTI grants not related to the equipment requested here; therefore, they cannot be listed on this application. Together, we consist of faculty members representing the departments of Chemical Engineering, Mechanical Engineering, Biology, and Psychiatry and Behavioral Neurosciences. Our research programs encompass drug development, formulation and delivery, including a nanotechnology platform for intranasal drug delivery targeting central nervous system disorders. The real--time qPCR instrument will be used for the quantification of gene expression for genes that are altered in disease states and regulated by novel drugs and drug formulations we have developed. Experiments measuring gene expression will be performed in cellular, worm and pre-clinical rodent models of various brain disorders. Similar equipment is not available in nearby laboratories at McMaster; therefore, procurement of this instrument is essential for conducting our experiments and the advancement of our federally funded collaborative research programs. The availability of this instrument will significantly advance our innovative research programs and promote the collaboration of experiments with the aim of developing novel drug compounds and improving drug delivery to the brain. This will ultimately result in increased therapeutic efficacies aimed to reach clinical stages for the treatment of neurodegenerative disorders. Furthermore, our research will significantly contribute to the field's understanding of novel neurotrophic factors and their molecular mechanisms. Acquiring this instrument will immediately benefit all our students through an increase in accessibility and experience using current industry-standard machines. Ultimately, the results obtained from our research programs will significantly benefit Canada and the international community with regards to knowledge translation and dissemination of the availability of new therapeutics for the treatment of brain disorders.**