Developing High Speed Scanning Electrochemical Microscopy of Biological Substrates

JANINE MAUZEROLL (MCGILL UNIVERSITY; PIN: 290829)***提案摘要***在我们的研究中，我们使用电化学来研究化学和生物系统。我们感兴趣的是开发基于电化学的成像方法，可以用作诊断工具。我们的方法是多方面的，因为它结合了生物学，分析化学，材料科学和显微镜的大量专业知识。******在即将到来的资助期内，我们将利用我们过去六年在三个相互关联的系统上所取得的进展。***(I)我们将开发一种高速电分析方法，以量化和绘制与非均匀电化学活性表面（腐蚀合金，电池）发生的关键氧化还原过程相关的材料通量。这种尖端的电化学成像技术有望作为能够追踪关键癌症相关转运体泵活性的诊断分析方法。***（二）基于氧化还原活性磷脂设计智能给药纳米材料。我们的目标是探索这类新磷脂的电化学和自组装。***（三）我们将继续发展分析电化学成像策略来研究肿瘤细胞的多药耐药。我们的目标是开发一种定量的多药耐药试验，能够评估封装在II.**中开发的纳米材料中的抗癌药物的效果

JANINE MAUZEROLL (MCGILL UNIVERSITY; PIN: 290829)***Summary of Proposal***In our research we use electrochemistry to study chemical and biological systems. We are interested in developing electrochemical based imaging methods that can be used as diagnostic tools. Our approach is multifaceted as it combines considerable expertise in biology, analytical chemistry, material sciences, and microscopy.******In the coming grant period, we will capitalize on our progress over the past 6 years working on 3 interrelated systems. ***(I) We will develop a high-speed electroanalytical methodology to quantify and map material fluxes related to key redox processes occurring at surfaces with heterogeneous electrochemical activity (corroding alloys, cells). Such cutting-edge electrochemical imaging techniques are promising as diagnostic assays capable of tracking the activity of key cancer-related transporter pumps.***(II) We will design smart drug delivery nanomaterials based on redox active phospholipids. Our goal is to explore the electrochemistry and self-assembly of this new class of phospholipids.***(III) We will pursue development of analytical electrochemical imaging strategies to study multidrug resistance of patterned cancer cells. Our goal is to develop a quantitative multidrug resistance assay capable of evaluating the effects of anticancer agents encapsulated in the nanomaterials developed in II.**