Ultrahigh performance liquid chromatography - triple quadruple mass spectrometer for quantitative chemical and biochemical analysis

我们请求资金购买一台超高性能液相色谱三重四极杆质谱仪(UHPLC-TQMS)，以实现准确的化学定量。UHPLC-TQMS是一个成熟的分析平台，提供极其灵敏、可重复性和高精度的分子测量，具有极强的稳健性，这是各种研究应用迫切需要的。该LC-MS在UBC化学质谱学设施中运行和维护，将有助于满足UBC各地研究人员对定量化学、代谢物和蛋白质分析的高需求和未得到满足的需求。申请者来自化学系、动物学系和土地与食品系统学系，在代谢组学和质谱学(陶欢)、天然产物生物合成(凯瑟琳·瑞恩)、蛋白质生物化学(苏珊娜·斯特劳斯)、实时化学反应监测(Jason Hein)、化学生物标志物(David Chen)、有机污染物的痕量水平(Allan Bertram)、比较生理学(Katie Matt)和分子食品安全(Barbara Stefanska)等领域进行重要研究。除了在(1)定向代谢组学、(2)微量化学物质的检测和量化以及(3)蛋白质定量方面创造新的和维持现有的研究、合作和资助机会，该仪器还将为至少91名高素质人员(HQP)提供实践培训。化学系已将TQMS视为一项关键采购，并正在为这项申请提供大量配套资金。拟议的仪器将是未来几十年的重要主力，通过培训HQP并通过在几个化学领域和其他领域培养卓越的研究成果，为大学及其员工增加巨大价值。

We request funds to purchase an ultrahigh performance liquid chromatography triple quadrupole mass spectrometer (UHPLC-TQMS) for accurate chemical quantification. UHPLC-TQMS is a well-established analytical platform that provides extremely sensitive, reproducible, and precise molecular measurements with great robustness, which is urgently needed for a variety of research applications. Operated and maintained in the UBC Chemistry Mass Spectrometry Facility, this LC-MS will help address the high and unmet demand for quantitative chemical, metabolite, and protein analysis by researchers across UBC. Applicants are from the Departments of Chemistry, Zoology, and the Faculty of Land & Food Systems, and conduct important research in the areas of metabolomics and mass spectrometry (Tao Huan), natural product biosynthesis (Katherine Ryan), protein biochemistry (Suzana Straus), real-time chemical reaction monitoring (Jason Hein), chemical biomarkers (David Chen), trace levels of organic pollutants (Allan Bertram), comparative physiology (Katie Marshall) and molecular food safety (Barbara Stefanska). In addition to creating new and maintaining existing research, collaboration and funding opportunities for (1) targeted metabolomics, (2) detection and quantification of trace levels of chemicals, and (3) protein quantification, the instrument will also provide hands-on training for at least 91 highly qualified personnel (HQP). The Department of Chemistry has deemed a TQMS as a critical purchase and is supporting this application with substantial matching funds. The proposed instrument will be an important workhorse for the next several decades, adding great value to the university and its employees through the training of HQP and by fostering research excellence in several areas of chemistry and beyond.