Multimode microplate reader with imaging for versatile analyses in immunology, microbiology, biochemistry and environmental toxicology

我们正在申请资金，用于集成显微镜和成像的多模式酶标仪。多模式读板器具有众多的通用应用，使其广泛使用，并成为许多类型研究的必要工具。功能方面的最新进展极大地扩展了多模式酶标仪可以执行的测量和技术范围。由于这种广泛的多功能性，多模式酶标仪是必不可少的许多研究应用在我们的研究小组。例如，我们需要这种仪器来检查肠道微生物，饮食和运动对免疫系统的影响，用于使用siRNA开发治疗性基因沉默策略，用于环境研究检查藻类在水生生态系统中的作用和环境污染物的生物转化，用于开发非致命策略用于五大湖的环境影响监测，以及用于分析细胞相互作用，所述细胞相互作用是检查用于骨和神经组织的工程化组织支架的生物相容性所必需的。 目前，我们完全依赖于一个过时的读板器，其零件不再可用，也不再支持维修。 虽然我们已经完成了最近的维修工作，但很明显，酶标仪很快就会无法修复，使我们无法进行日常进行的许多重要测定和测量。 该仪器是必不可少的，以保持我们的研究进展，并支持在高度协作的应用生物科学研究环境中的协同研究在安大略技术。 使用具有成像能力的当前模型多模读取器将为博士后学员以及研究生和本科生提供各种技术的市场技能，包括不断增加的生物测定和生物标志物定量阵列。该仪器非常适合分析各种细胞类型和生物体，从细菌和藻类到鱼类胚胎和人类细胞，涵盖了我们高度合作的研究项目的研究重点。将集中使用具有成像功能的多模式酶标仪，为具有市场竞争力的HQP提供跨学科实践培训的最佳机会，以满足加拿大对生物技术，制药和农业食品行业以及环境监测相关专业知识日益增长的需求。

We are requesting funds for a multimode microplate reader with integrated microscopy and imaging. Multimode plate readers have numerous and versatile applications, making them widely used and essential tools for many types of research. Recent advances in functionality have greatly expanded the range of measures and techniques that multimode microplate readers can perform. Due to this broad versatility, a multimode microplate reader is essential for numerous research applications in our research groups. For example, we require this instrument to examine the impact of gut microbes, diet and exercise on the immune system, for development of therapeutic gene-silencing strategies using siRNA, for environmental research examining roles of algae in aquatic ecosystems and biotransformation of environmental contaminants, for development of non-lethal strategies for environmental effects monitoring in the Great Lakes, and for analysis of cellular interactions necessary to examine the biocompatibility of engineered tissue scaffolds for bone and nervous tissue. At present, we are completely reliant on an obsolete plate reader for which parts are no longer available and repair is no longer supported. While we have completed recent repairs ourselves, it is evident that the microplate reader will soon be beyond repair, leaving us unable to perform numerous essential assays and measures that we conduct on a daily basis. This instrument is essential to maintain our research progress and to support research synergies in the highly collaborative Applied Bioscience research environment at Ontario Tech. Working with a current model multimode reader with imaging ability will provide marketable skills to post-doctoral trainees and to graduate and undergraduate students in varied techniques including an ever-increasing array of bioassays and biomarker quantification. This instrument is ideal for analysis of a wide range of cell types and organisms, from bacteria and algae to fish embryos and human cells, encompassing the research foci of our highly collaborative research programs. The multimode microplate reader with imaging will be used intensively, providing cutting edge opportunities for interdisciplinary hands-on training of highly competitive HQP with marketable skills to meet growing needs in Canada for expertise relevant for biotechnology, pharmaceutical and agri-food industries and in environmental monitoring.