Replacement documentation system for quantifying protein levels and other biological applications.
Replacement documentation system for quantifying protein levels and other biological applications.
批准号:
RTI-2023-00048
负责人:
Todd, Christopher
金额:
$3.51万
依托单位国家:
加拿大
项目类别:
Research Tools and Instruments
财政年份:
2022
资助国家:
加拿大
项目状态:
已结题
起止时间:
2022-01-01 至 2023-12-31
中文摘要
通过蛋白质印迹法可视化和定量蛋白质样品的丰度是现代生物化学和分子生物学中的基本和标准技术。使用化学发光或荧光代替样品的比色检测现在是标准方案。虽然荧光成像和定量可以使用胶片、显影剂和扫描软件来完成,但使用凝胶记录系统避免了对消耗品的需要和化学废物的产生,现在也是标准的。RTI的应用是用能够进行荧光和荧光检测的Bio-Rad ChemiDoc MP系统取代过时的凝胶记录系统。这些设备将定期用于支持共同申请人(托德,魏,王,火腿)实验室的研究和HQP培训,以及萨斯喀彻温大学合作科学研究大楼多个学术单位的其他用户。
英文摘要
Visualizing and quantifying abundance of protein samples via western blotting is a fundamental and standard technique in modern biochemistry and molecular biology. The use of chemiluminescence or fluorescence instead of colorimetric detection of samples is now a standard protocol. While chemiluminescent imaging and quantification can be accomplished using film, a developer, and scanning software the use of a gel documentation system that obviates the need for the consumables and generation of chemical waste is also now standard. The RTI application is to replace an obsolete gel documentation system with a Bio-Rad ChemiDoc MP system capable of chemiluminescent and fluorescent detection. The equipment will be used regularly in support of research and HQP training in the labs of the co-applicants (Todd, Wei, Wang, Ham) as well as other users from multiple academic units in the Collaborative Science Research Building at the University of Saskatchewan.
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会议论文
Ureide metabolism in response to abiotic stress in plants
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批准号:RGPIN-2018-04251
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项目类别:Discovery Grants Program - Individual
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资助金额:$5.83万
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财政年份:2022
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负责人:Todd, Christopher
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依托单位:
Ureide metabolism in response to abiotic stress in plants
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批准号:RGPIN-2018-04251
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项目类别:Discovery Grants Program - Individual
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资助金额:$2.91万
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财政年份:2021
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负责人:Todd, Christopher
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依托单位:
Ureide metabolism in response to abiotic stress in plants
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批准号:RGPIN-2018-04251
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项目类别:Discovery Grants Program - Individual
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资助金额:$2.91万
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财政年份:2020
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负责人:Todd, Christopher
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依托单位:
Ureide metabolism in response to abiotic stress in plants
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批准号:RGPIN-2018-04251
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项目类别:Discovery Grants Program - Individual
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资助金额:$2.91万
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财政年份:2019
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负责人:Todd, Christopher
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依托单位:
Ureide metabolism in response to abiotic stress in plants
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批准号:RGPIN-2018-04251
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项目类别:Discovery Grants Program - Individual
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资助金额:$2.91万
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财政年份:2018
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负责人:Todd, Christopher
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依托单位:
Ureide metabolism and abiotic stress in plants
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批准号:327190-2013
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项目类别:Discovery Grants Program - Individual
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资助金额:$2.11万
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财政年份:2017
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负责人:Todd, Christopher
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依托单位:
Ureide metabolism and abiotic stress in plants
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批准号:327190-2013
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项目类别:Discovery Grants Program - Individual
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资助金额:$2.11万
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财政年份:2015
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负责人:Todd, Christopher
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依托单位:
Ureide metabolism and abiotic stress in plants
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批准号:327190-2013
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项目类别:Discovery Grants Program - Individual
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资助金额:$2.11万
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财政年份:2014
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负责人:Todd, Christopher
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依托单位:
UV-Visible Spectrophotometer for Plant and Fungi Cellular and Molecular Biology Research
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批准号:458704-2014
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项目类别:Research Tools and Instruments - Category 1 (<$150,000)
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资助金额:$1.77万
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财政年份:2013
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负责人:Todd, Christopher
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依托单位:
Ureide metabolism and abiotic stress in plants
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批准号:327190-2013
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项目类别:Discovery Grants Program - Individual
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资助金额:$2.11万
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财政年份:2013
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负责人:Todd, Christopher
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依托单位:
Regulation of ureide metabolism in legumes
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批准号:327190-2007
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项目类别:Discovery Grants Program - Individual
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资助金额:$2.31万
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财政年份:2011
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负责人:Todd, Christopher
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依托单位:
Regulation of ureide metabolism in legumes
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批准号:327190-2007
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项目类别:Discovery Grants Program - Individual
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资助金额:$2.31万
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财政年份:2010
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负责人:Todd, Christopher
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依托单位:
Regulation of ureide metabolism in legumes
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批准号:327190-2007
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项目类别:Discovery Grants Program - Individual
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资助金额:$2.31万
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财政年份:2009
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负责人:Todd, Christopher
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依托单位:
Reach-in plant growth chamber for plant metabolism research
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批准号:375998-2009
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项目类别:Research Tools and Instruments - Category 1 (<$150,000)
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资助金额:$1.43万
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财政年份:2008
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负责人:Todd, Christopher
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依托单位:
Regulation of ureide metabolism in legumes
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批准号:327190-2007
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项目类别:Discovery Grants Program - Individual
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资助金额:$2.31万
-
财政年份:2008
-
负责人:Todd, Christopher
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依托单位:
Regulation of ureide metabolism in legumes
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批准号:327190-2007
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项目类别:Discovery Grants Program - Individual
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资助金额:$2.31万
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财政年份:2007
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负责人:Todd, Christopher
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依托单位:
Chromatography refrigerator and freezers for plant molecular biology
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批准号:345606-2007
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项目类别:Research Tools and Instruments - Category 1 (<$150,000)
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资助金额:$1.35万
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财政年份:2006
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负责人:Todd, Christopher
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依托单位:
Ureide breakdown and nitrogen fixation in soybean in response to drought
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批准号:252725-2002
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项目类别:Postdoctoral Fellowships
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资助金额:$3.02万
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财政年份:2003
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负责人:Todd, Christopher
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依托单位:
Ureide breakdown and nitrogen fixation in soybean in response to drought
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批准号:252725-2002
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项目类别:Postdoctoral Fellowships
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资助金额:$2.55万
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财政年份:2002
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负责人:Todd, Christopher
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依托单位:
PGSB
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批准号:232883-2000
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项目类别:Postgraduate Scholarships
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资助金额:$1.39万
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财政年份:2001
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负责人:Todd, Christopher
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依托单位:
海外基金