Understanding the Biochemical Characteristics of Canine Saliva

蛋白质组学研究已经在兽医科学中开始；具体来说，蛋白质组在细胞、分泌物和农场动物组织中的探索呈指数增长。然而，伴侣动物（如狗）的蛋白质组还没有被详细研究过。尽管在大多数脊椎动物中，唾液在口腔内平衡的调节中起着重要作用，但对不同哺乳动物物种唾液组成的研究还不够。狗的唾液在组成、生理和生物化学方面的研究很少。基于对狗嘴里的唾液成分所知甚少，我们可以强调人类和狗唾液之间的显著差异。缺乏对人类和狗唾液之间这种关键差异的关注以及狗唾液基础研究的差距可能部分解释了为什么基于人类唾液开发的治疗方法在狗身上效果不佳。在影响狗的疾病中，牙石和牙周炎是常见的。在自然界中，对这些疾病的主要保护是唾液成分假设：狗的唾液具有独特的组成和生物化学特征，负责促进口腔内稳态。我的短期目标是了解狗的唾液生物化学。在这里，该资助包括3个新的研究领域：目标1：狗唾液中的蛋白质组和肽球的研究；目标2：鉴定狗唾液中的磷酸化蛋白质组及其磷酸化位点；目标3：测定狗唾液的唾液流速、无机成分、pH和缓冲能力。研究计划：收集狗的唾液。此外，唾液将进行蛋白质组学和肽组学分析。所有获得的质谱数据将与一个综合的狗、哺乳动物和人类蛋白质数据库进行分析（目的1）。最后，将使用磷酸化蛋白质组方法来鉴定磷酸化蛋白及其磷酸化位点（目的2）。此外，将使用分析方法分析样品的离子组成、pH值和缓冲容量（目标3）研究的相关性：我建议进行前5年的开创性科学探索，以全面表征狗唾液。第一次，我计划表征狗唾液的肽和磷蛋白组。作为一个长期的目标，我将利用这个申请产生的研究成果来继续我的研究项目，我计划在那里了解狗的牙石和牙周病的形成。此外，我相信这种组学研究的总体结果有可能通过识别狗口腔和全身疾病的生物标志物，开辟与兽医科学直接相关的研究新途径——这是一个对宠物私营部门具有重大吸引力的研究领域，我计划通过使用NSERC-ALLIANCE赠款和CHRP赠款等更具转化性的资助工具参与其中

Proteomics studies have been initiated in veterinary science; specifically, the proteome exploration in cells, secretions, and tissues of farm animals has increased exponentially. Still, the proteome of companion animals, like dogs, has not been studied in detail. Despite the weight of saliva in the regulation of oral homeostasis in most vertebrates, insufficient studies have been conducted to investigate the salivary composition of different mammal species. Dog saliva is very poorly studied in terms of composition, physiology, and biochemistry. Based on the little that is known about the salivary composition present in the dog mouth, we can highlight significant differences between human and dog saliva. The lack of attention to such critical variations between human and dog saliva and the gap of foundational studies in dog saliva may partially explain why the use of therapeutics developed based on human saliva are not efficient in dogs. Among the diseases that affect dogs, dental calculus and periodontitis are common. In nature, the main protection against these diseases are the salivary components Hypothesis: Dog's saliva has unique composition and biochemistry characteristics that are responsible for promoting oral cavity homeostasis My short-term goals are to understand the dog saliva biochemistry. Here, this grant includes 3 novel areas of investigation: Aim 1: Investigation of the proteome and peptidome in dog saliva Aim 2: Identification of phosphoproteome and their phosphorylated sites in dog saliva Aim 3: Determination of salivary flow rate, inorganic composition, pH and buffer capacity in dog saliva Research Plan: Saliva from dogs will be collected. In addition, saliva will be subjected to proteomic and peptidomic analysis. All acquired mass spectrum data will be analyzed against a comprehensive dog, mammalian and human protein databank (Aim 1). Finally, a phosphoproteome approach will be used to identify the phosphoproteins and their phosphorylation sites (Aim 2). Also, samples will be analyzed for ion composition, pH, and buffer capacity using analytical methods(Aim 3) Relevance of the study: I am proposing the first 5 years of a pioneering scientific exploration towards a comprehensive characterization of the dog saliva. For the first time, I plan to characterize the peptidome and phosphoproteome of dog saliva. As a long-term goal, I will use the research findings generated from this application for continuing my research program where I plan to understand the formation of dental calculus and periodontal disease in dogs. Moreover, I believe that the overall outcome of such OMICS studies has the potential to open novel avenues of investigations with direct relevance to veterinary sciences by identifying biomarkers for dogs oral and systemic diseases - a research area with significant appeal with the Pet Private-Sector where I plan to engage by using a more translational grant tool like NSERC-ALLIANCE grant and CHRP grant