Understanding the Biochemical Characteristics of Canine Saliva

了解犬唾液的生化特征

• 批准号: RGPIN-2020-06119
• 负责人: Siqueira, Walter
• 金额: $ 2.4万
• 依托单位: University of Saskatchewan
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=717843
• 关键词: Understanding; Biochemical; Characteristics; Canine; Saliva

Proteomics studies have been initiated in veterinary science; specifically, the proteome exploration in cells, secretions, and tissues of farm animals has increased exponentially. Still, the proteome of companion animals, like dogs, has not been studied in detail. Despite the weight of saliva in the regulation of oral homeostasis in most vertebrates, insufficient studies have been conducted to investigate the salivary composition of different mammal species. Dog saliva is very poorly studied in terms of composition, physiology, and biochemistry. Based on the little that is known about the salivary composition present in the dog mouth, we can highlight significant differences between human and dog saliva. The lack of attention to such critical variations between human and dog saliva and the gap of foundational studies in dog saliva may partially explain why the use of therapeutics developed based on human saliva are not efficient in dogs. Among the diseases that affect dogs, dental calculus and periodontitis are common. In nature, the main protection against these diseases are the salivary components Hypothesis: Dog's saliva has unique composition and biochemistry characteristics that are responsible for promoting oral cavity homeostasis My short-term goals are to understand the dog saliva biochemistry. Here, this grant includes 3 novel areas of investigation: Aim 1: Investigation of the proteome and peptidome in dog saliva Aim 2: Identification of phosphoproteome and their phosphorylated sites in dog saliva Aim 3: Determination of salivary flow rate, inorganic composition, pH and buffer capacity in dog saliva Research Plan: Saliva from dogs will be collected. In addition, saliva will be subjected to proteomic and peptidomic analysis. All acquired mass spectrum data will be analyzed against a comprehensive dog, mammalian and human protein databank (Aim 1). Finally, a phosphoproteome approach will be used to identify the phosphoproteins and their phosphorylation sites (Aim 2). Also, samples will be analyzed for ion composition, pH, and buffer capacity using analytical methods(Aim 3) Relevance of the study: I am proposing the first 5 years of a pioneering scientific exploration towards a comprehensive characterization of the dog saliva. For the first time, I plan to characterize the peptidome and phosphoproteome of dog saliva. As a long-term goal, I will use the research findings generated from this application for continuing my research program where I plan to understand the formation of dental calculus and periodontal disease in dogs. Moreover, I believe that the overall outcome of such OMICS studies has the potential to open novel avenues of investigations with direct relevance to veterinary sciences by identifying biomarkers for dogs oral and systemic diseases - a research area with significant appeal with the Pet Private-Sector where I plan to engage by using a more translational grant tool like NSERC-ALLIANCE grant and CHRP grant

蛋白质组学研究已开始在兽医科学;具体地说，蛋白质组学探索细胞，分泌物，和组织的农场动物已经成倍增加。尽管如此，伴侣动物的蛋白质组，如狗，还没有被详细研究。尽管在大多数脊椎动物中唾液的重量在口腔内稳态的调节中，但对不同哺乳动物物种的唾液组成的研究还不够。狗的唾液在成分、生理学和生物化学方面的研究非常少。基于对狗口中唾液成分的了解很少，我们可以突出人和狗唾液之间的显着差异。缺乏对人和狗唾液之间的这种关键变化的关注以及对狗唾液的基础研究的差距可能部分解释了为什么基于人唾液开发的治疗方法在狗中无效。在影响狗的疾病中，牙石和牙周炎是常见的。在自然界中，主要的保护措施，对这些疾病的唾液成分 假说：狗的唾液具有独特的成分和生物化学特征，负责促进口腔内环境的稳定 我的短期目标是了解狗唾液的生物化学。在这里，这笔赠款包括3个新的调查领域： 目的1：研究犬唾液蛋白质组和肽组 目的2：鉴定犬唾液磷酸化蛋白质组及其磷酸化位点 目的3：测定犬唾液的流速、无机成分、pH和缓冲能力 研究计划：采集犬的唾液。此外，将对唾液进行蛋白质组学和肽组学分析。将根据综合犬、哺乳动物和人蛋白质数据库分析所有采集的质谱数据（目标1）。最后，磷酸化蛋白质组学方法将用于鉴定磷酸化蛋白及其磷酸化位点（目的2）。此外，将使用分析方法分析样品的离子组成、pH值和缓冲容量（目标3） 研究的相关性：我建议在前5年进行开创性的科学探索，以全面表征狗的唾液。我计划第一次对狗唾液的肽组和磷酸化蛋白质组进行表征。作为一个长期的目标，我将使用从这个应用程序中产生的研究结果继续我的研究计划，我计划了解狗的牙结石和牙周病的形成。此外，我相信，这种OMICS研究的总体结果有可能通过确定犬口腔和全身疾病的生物标志物，开辟与兽医科学直接相关的新的研究途径-这是一个对宠物私营部门具有重大吸引力的研究领域，我计划通过使用更多的翻译资助工具，如NSERC-联盟资助和CHRP资助，参与其中