A Single Cell Isolating System for Genomics Applications, Phase 1 I2I

UBC Isolatrix打印机将是一种高性价比的解决方案，可满足众多使用案例中尚未满足的单电池制备需求。目前的单细胞分离方法包括随机封装法和显微操作法。泊松分布下的随机封装具有较低的填充因子，具有大量的空井或液滴，而目前使用微操作的工具可以提高填充因子，但对单元的剪切力较大，或处理时间较长。我们的系统是一款高通量、基于喷墨的单电池打印机，采用了专门的光学和机器视觉技术。我们已经建立了Isolatrix技术的基本参数，并通过测试证明该概念将在单细胞点胶中发挥预期作用。单颗粒分配率大于90%，每个分配颗粒的速度小于2秒。该系统被设计成与从标准微滴定板到微孔芯片的一系列基板尺寸兼容。在这个第一阶段的项目中，我们将通过我们的市场评估解决基本问题，并通过与我们在BC癌症研究中心的合作伙伴验证该技术，我们将推进该技术并展示其在单细胞基因组研究中的应用，从而使目前受到单细胞分离所需时间限制的项目得以实现。

The UBC Isolatrix printer will be a cost-effective solution to fill an unmet need for single-cell preparation for numerous use cases. Current methods for single-cell isolation include random encapsulation and micromanipulation. Random encapsulation following the Poisson distribution gives a low fill factor with a large number of empty wells or droplets, while current tools using micromanipulation can increase the fill factor but with high shear force on cells, or high processing time. Our system is a high-throughput, inkjet based single-cell printer enabled by specialized optics and machine vision. We have already established the basic parameters of our Isolatrix technology, and demonstrated through testing that the concept will function for its intended purpose in single cell dispensing. Single-particle dispense rates of greater than 90% are achieved at a speed of less than 2 seconds per dispensed particle. The system is designed to be compatible with a range of substrate footprints ranging in size from standard microtiter plates to microwell chips. In this Phase 1 project we will address the essential questions asked through our Market Assessment, and through validation of the technology with our partner at the BC Cancer Research Centre, we will advance the technology and demonstrate its application for single-cell genomics studies, enabling projects that are currently limited by the time required for single cell isolation.