Development of a process to produce proteases and lipases by fermentation of compost leachate and agri-food wastes using Bacillus sp. and Rhizobium sp

萨尼马克集团成立于1969年，位于加拿大维多利亚维尔，是生产和销售工业、农业食品、游泳和水疗应用清洁和消毒产品的领导者。Sani Marc始终关注环境保护，通过优化操作条件和减少对环境的影响来满足市场对清洁和消毒产品的需求。同样，Sani Marc集团对通过发酵农业食品残渣（如堆肥渗滤液和乳清）获得的“绿色”酶的生产感兴趣。感兴趣的酶是由芽孢杆菌和根瘤菌产生的蛋白酶和脂肪酶。利用芽孢杆菌生产这些酶是有充分记录的，利用农业食品残留物开发生物工艺是非常可行的。在使用根瘤菌生产脂肪酶的情况下，该项目将验证它是否在技术上和经济上可行。该项目将包括发酵和酶提取纯化的优化。酶的配方将在稳定性和作为生产清洁产品的原料方面进行测试。该项目聚焦于零废物的概念，通过探索液体废物的增值。绿色酶的生产将巩固赛尼马克集团作为环保企业在北美的领先地位。该项目将允许开发新的环保产品，这些产品可以获得有机认证。

Sani Marc Group, founded in 1969 at Victoriaville, QC, Canada, is a leader in the manufacture and distribution of cleaning and sanitizing products for industry, agri-food, swimming and spa applications. Always concerned about the protection of the environment, Sani Marc arises to meet the needs on the market in cleaning and sanitizing products by optimizing operating conditions and reducing environmental impact.Likewise, the Sani Marc Group is interested in the production of 'green' enzymes obtained by the fermentation of agri-food residues, such as compost leachate and whey. The enzymes of interest are proteases and lipases produced by Bacillus sp and Rhizobium sp. The production of these enzymes using Bacillus is well documented and the development of a bioprocesses using agri-food residues is highly feasible. In the case of using Rhizobium to produce lipases, the project will let to verify if it would be techno-economically feasible.The project will include the optimization of fermentation and enzyme extraction-purification. The formulations of enzymes will be tested in terms of stability and as raw materials for producing cleaning products. The project focuses the zero-waste concept, by exploring the valorization of liquid wastes. The production of green enzymes will consolidate the leading position of Sani Marc Group in North America as an environmentally responsible enterprise. This project will allow the development of new eco-friendly products which could be organic certified.