短直链淀粉自组装纳米颗粒形成机理与多酚类物质多元运载体系的构建

本项目以天然高分子淀粉为研究对象，以短直链淀粉分子自组装、功能化为基础，以构建稳定的、智能释放型多酚类多元纳米运载体系为导向，对淀粉纳米颗粒形成机理及运载体系中各组分与载体特性的构效关系和释放机理等基础科学问题进行系统研究。项目借助原位冷冻透射电镜、小角X散射等对晶核形成、生长规律和机理进行研究，利用分子仿真技术建立纳米颗粒形成动力学模型。采用自组装法构建包埋、吸附、包埋-吸附复合、pH值和淀粉酶双敏感等四种类型多酚类多元纳米运载体系，探究纳米颗粒与乳化剂、多糖（蛋白）等相互作用机制。阐释组分比例、分子结构、电荷对体系中多酚的装载率、稳定性、吸附动力学、抗氧化活性影响及释放规律，揭示体系控释机理及纳米载体对多酚活性的作用机制。并结合体内外实验建立多酚释放动力学模型，提高多酚类活性物质的稳定性和生物利用率。

In this project, a natural polymer-starch will be used as study material to comprehensively investigate the formation mechanism of starch nanoparticles. The study is based on self-assembly and manipulation of short-chain amylose with the target of establishing stable and intelligent release polyphenols nano-multiple delivery systems. This project will explore the rules and mechanisms of nucleation and crystal growth by using in situ cryo-transmission electron microscope, small angle scattering and so on, and establish nanoparticle formation dynamic model based on molecular simulation technology. Four types of polyphenols nano-multiple delivery systems such as encapsulation, adsorption, encapsulation-adsorption complex and double sensitive to pH and amylase types will be self-assembly established. This project will explore interaction mechanisms among starch nanoparticles, emulsifiers and polysaccharides (proteins) and the effect of components proportions, molecular structure and charge on the polyphenols loading capacity, stability, adsorption kinetics, antioxidant activity and rules of release. This is aimed to reveal the mechanisms of controlled release of carrier systems and activity of nano-carrier on polyphenols. The in vitro and in vivo releasing properties of polyphenols and their releasing mechanisms will be also determined. All of these researches are aimed to increase the stability and bioavailability of polyphenols.