信息素受体多等位基因在田头菇属真菌生物种识别中的作用研究

真菌交配型位点由信息素及其受体组成，控制着有性生殖过程。交配型基因研究多集中在位点组成方面，有关物种内信息素受体群体组成特征研究很少报道。本项目拟以西南地区分布的田头菇属真菌为研究对象，建立确定生物学种类的菌株群体，根据前期已获得的信息素受体保守区片段，DNA walking 获得基因全长，扩增获得所有供试菌株的受体基因信息；对已得到6个差异片段的A.salicacola和A.aegerita菌株，分别克隆获得信息素受体全长，同样设计引物扩增供试菌株，比较物种之间信息素受体的差异；用Real-time PCR的方法，检测信息素受体在菌株中的的表达活性。系统研究物种信息素受体编码基因组成特征，探索其在生物学物种鉴定中的作用，期望以此为模式，为其它真菌物种的有性生殖研究提供借鉴，完善真菌遗传的分子研究体系。

Mating type loci are composed of genes encoding pheromones and corresponding receptors, which control the sexual reproduction of fungi. Most studies were focused on the organization of loci, but few reports were involved in the group trait of pheromone receptors in the species. In this plan, some fungi of Agrocybe genus are used as the target strains for constructing the population with determination of "biological species". Based on the conserved segments amplified in the previous study, the complete gene sequences are obtained using the DNA walking method, and the specific primers are designed to amplify all pheromone receptor genes in tested strains; For the A.salicacola and A.aegerita strains ocntaining six differenti segments, which were already cloned, their complete genes are cloned and specific primers are used to to amplify all strains for comparing the divergence of pheromone receptor in different species; The Real-time PCR method is employed to test the activity of pheromone receptor in test strains. The above studies are used to the trait of genes encoding pheromoe receptor, explore the application of gene informaiton on identificaton of biological species, and is expected for providing reference on the sexual studies of other fungi to improve the molucular system of heredity of fungi.