拟康氏木霉合成的康宁霉素促进植物根系生长的作用与机制

木霉是一类重要的半知菌亚门真菌资源，广泛地用于工业用酶的生产和植物病害的生物防治。木霉具有直接促进植物生长的作用,但木霉-植物相互作用的研究主要集中在木霉诱导植物抗性上，其对植物生长发育的直接促进作用及机制尚缺乏深入研究。我们的前期研究发现，拟康氏木霉SMF2及其合成的peptaibols类抗菌肽-康宁霉素除对植物病原微生物具有很好的拮抗作用外，还能促进植物的生长发育。本研究拟以木霉SMF2为出发菌株，以拟南芥等植物为研究材料，研究拟康氏木霉及其所产生的康宁霉素促进植物根系生长发育的作用与机制。首先通过构建康宁霉素缺失的木霉突变株，比较突变菌株和出发菌株对植物生长发育的影响，明确康宁霉素的产生是拟康氏木霉促进植物生长发育的机制之一。进而系统研究康宁霉素处理对植物根系生长发育的影响，阐明康宁霉素促进植物根系生长发育的机制。该研究有助于深入认识木霉-植物之间的相互作用，更好地利用木霉资源。

Trichoderma species are important fungal resouses belonging to Deuteromyeotina, which are common used for industrial enzymes production and plant disease control.Many studies found that Trichoderma has direct effect on plant growth, but the mechanism remains poorly understood. Our primary studies found that Trichoderma pseukoningii SMF2 and its secondary metabolite-Trichokonins not only have the antigonistic effect on plant pathogens,but also promote plant growth. In this study, we will study the effect and mechanism of Trichokonins from Trichoderma pseukoningii SMF2 on plant root growth and development.After construct Trichokonins-deletion strain, we will compare the effect of deletion mutant and the original strain on plant root growth to make clear that Trichokonins production is one of mechanisms for plant growth promoting effect of Trichoderma pseukoningii SMF2.Then we will study the effects of Trichokonins on plant root growth and development systemly,and thus elucidate the plant growth promoting mechamism of Trichonins. This study will contribute to further understanding interaction between Trichoderma and plant, and lay the theoretical foundation for better use Trichoderma.