The fate of Osterix-expressing mesenchymal cells in dental root formation and maintenance.
The fate of Osterix-expressing mesenchymal cells in dental root formation and maintenance.
复制标题
在牙齿根形成和维持中表达卵形质细胞的命运。
DOI：
10.1111/ocr.12167
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发表时间：
2017-06
影响因子：
3.1
通讯作者：
Ono W
中科院分区：
文献类型：
--
作者：
Takahashi A;Ono N;Ono W
表达Osterix(OSX)的间充质细胞是牙根形成细胞的祖细胞。这项研究的目的是通过谱系追踪实验来揭示OSX表达细胞在根形成过程中和之后的命运。为了揭示表达OSX的牙间充质祖细胞的命运，我们利用了他莫昔芬诱导的Cre报告系统。R26R-td番茄小鼠在出生后第3天(P3)灌胃他莫昔芬(0.1 mg/只)。在这个系统中，在P3(OSX-P3)细胞中表达OSX的细胞进行重组，它们及其后代继续永久表达番茄红色荧光蛋白。在从P4到P116的连续时间点解剖下颌骨，以研究OSX-P3细胞如何参与牙根的形成。冰冻切片上的番茄+细胞在荧光显微镜下成像。OSX-P3细胞及其传代细胞在牙根形成过程中分化为牙根和牙周组织的各种细胞，如成牙本质细胞、成牙骨质细胞、牙槽骨成骨细胞和牙周膜细胞。即使在牙根形成完成后，它们仍然存在于牙髓和牙髓中，为成牙本质细胞和成牙骨质细胞提供祖细胞。OSX表达细胞在牙根形成的整个过程中起着重要作用；它们的后代即使在牙根形成完成后仍继续对牙根的维持做出贡献。
Osterix (Osx)-expressing mesenchymal cells are progenitors for tooth root forming cells. The aim of this study was to reveal the fates of Osx-expressing cells during and after root formation using a lineage tracing experiment. To reveal the fates of Osx-expressing dental mesenchymal progenitors, we took advantage of tamoxifen-inducible Cre reporter system. Osx-creER; R26R-tdTomato mice received tamoxifen (0.1 mg/body) at postnatal day 3 (P3). In this system, Osx-expressing at P3 (Osx-P3) cells undergo recombination, and they and their descendants continue to express Tomato red fluorescence protein permanently. Mandibles were dissected at serial time points ranging from P4 to P116 to investigate how Osx-P3 cells participated in root formation. Tomato+ cells on frozen sections were imaged under fluorescence microscopy. Osx-P3 cells and their descendants differentiated into all kinds of cells that contributed to the root and periodontal tissues, such as odontoblasts, cementoblasts, alveolar bone osteoblasts and periodontal ligament (PDL) cells during root formation. Even after root formation was completed, they persisted in dental pulp and PDL to provide progenitor cells for odontoblasts and cementoblasts. Osx-expressing cells play important roles in the entire processes of tooth root formation; their progeny continue to contribute to maintenance of tooth root even after root formation is complete.
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影响因子：
64.5
作者：
Nakashima, K;Zhou, X;de Crombrugghe, B
通讯作者：
de Crombrugghe, B
DOI：
10.1359/jbmr.091103
发表时间：
2010-05
期刊：
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
影响因子：
--
作者：
Huang X;Xu X;Bringas P Jr;Hung YP;Chai Y
通讯作者：
Chai Y
影响因子：
2.7
作者：
Huang, Xiaofeng;Bringas, Pablo, Jr.;Slavkin, Harold C.;Chai, Yang
通讯作者：
Chai, Yang
影响因子：
4.3
作者：
Rios HF;Ma D;Xie Y;Giannobile WV;Bonewald LF;Conway SJ;Feng JQ
通讯作者：
Feng JQ
影响因子：
3.2
作者：
Lee, Tae-Yeon;Lee, Dong-Seol;Park, Joo-Cheol
通讯作者：
Park, Joo-Cheol