Periostin is essential for the integrity and function of the periodontal ligament during occlusal loading in mice.
Periostin is essential for the integrity and function of the periodontal ligament during occlusal loading in mice.
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DOI：
10.1902/jop.2008.070624
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发表时间：
2008-08
影响因子：
4.3
通讯作者：
Feng JQ
中科院分区：
文献类型：
--
作者：
Rios HF;Ma D;Xie Y;Giannobile WV;Bonewald LF;Conway SJ;Feng JQ
牙周膜（PDL）吸收和分配力的能力对于牙周稳态是必要的。这种适应性反应可能部分由关键分子骨膜素决定，骨膜素在咬合功能和炎症过程中维持 PDL 的完整性。 Periostin 主要在 PDL 中表达，与 βig-H3（转化生长因子-β [TGF-β] 诱导基因）高度同源。牙骨质、牙槽骨和骨膜素缺失小鼠的 PDL 在牙齿萌出后急剧恶化。本研究的目的是确定骨膜素在维持牙周组织功能完整性中的作用。对骨膜素缺失小鼠的牙周组织进行表征，然后卸下门牙。使用鼠 PDL 细胞系评估基质拉伸对骨膜素表达的影响。使用实时逆转录聚合酶链反应来量化骨膜素和TGF-β的mRNA水平。使用 TGF-β1 中和抗体来确定底物拉伸对骨膜素表达的影响是否是通过 TGF-β 介导的。在没有骨膜素的小鼠中，在牙齿萌出后观察到严重的牙周缺损。消除骨膜素缺失小鼠的咀嚼力可以挽救牙周缺陷。 48小时后，紧张的PDL细胞中的骨膜素表达增加了9.2倍，并且在体外TGF-β mRNA出现短暂增加。添加 2.5 ng/ml TGF-β1 中和抗体可阻断机械应力引起的骨膜素升高，这表明机械应变激活 TGF-β 具有潜在的自分泌作用并增加骨膜素表达。机械负荷保持足够的骨膜蛋白表达，以确保牙周组织响应咬合负荷的完整性。
The ability of the periodontal ligament (PDL) to absorb and distribute forces is necessary for periodontal homeostasis. This adaptive response may be determined, in part, by a key molecule, periostin, which maintains the integrity of the PDL during occlusal function and inflammation. Periostin is primarily expressed in the PDL and is highly homologous to βig-H3 (transforming growth factor-beta [TGF-β] inducible gene). Cementum, alveolar bone, and the PDL of periostin-null mice dramatically deteriorate following tooth eruption. The purpose of this study was to determine the role of periostin in maintaining the functional integrity of the periodontium. The periodontia from periostin-null mice were characterized followed by unloading the incisors. The effect of substrate stretching on periostin expression was evaluated using a murine PDL cell line. Real-time reverse transcription-polymerase chain reaction was used to quantify mRNA levels of periostin and TGF-β. TGF-β1 neutralizing antibodies were used to determine whether the effects of substrate stretching on periostin expression are mediated through TGF-β. Severe periodontal defects were observed in the periostin-null mice after tooth eruption. The removal of masticatory forces in periostin-null mice rescue the periodontal defects. Periostin expression was increased in strained PDL cells by 9.2-fold at 48 hours and was preceded by a transient increase in TGF-β mRNA in vitro. Elevation of periostin in response to mechanical stress was blocked by the addition of 2.5 ng/ml neutralizing antibody to TGF-β1, suggesting that mechanical strain activates TGF-β to have potential autocrine effects and to increase periostin expression. Mechanical loading maintains sufficient periostin expression to ensure the integrity of the periodontium in response to occlusal load.
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影响因子：
18.6
作者：
McCulloch, CAG;Lekic, P;McKee, MD
通讯作者：
McKee, MD
影响因子：
4.3
作者：
LEVY, GG;MAILLAND, ML
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影响因子：
3
作者：
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影响因子：
3
作者：
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通讯作者：
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影响因子：
50.3
作者：
Bao, SD;Ouyang, G;Wang, XF
通讯作者：
Wang, XF