The mitochondrion-located protein OsB12D1 enhances flooding tolerance during seed germination and early seedling growth in rice.
The mitochondrion-located protein OsB12D1 enhances flooding tolerance during seed germination and early seedling growth in rice.
复制标题
线粒体定位蛋白 OsB12D1 增强水稻种子萌发和早期幼苗生长过程中的洪水耐受性
DOI：
10.3390/ijms150813461
复制
发表时间：
2014-07-31
影响因子：
5.6
通讯作者：
Yang P
中科院分区：
文献类型：
--
作者：
He D;Zhang H;Yang P
B12 D属于Balem（大麦糊粉和胚）蛋白的功能未知亚组。在我们之前的水稻种子萌发研究中，我们鉴定了一个由LOC_Os7g41350编码的B12 D样蛋白（命名为OsB 12 D1）。OsB 12 D1是一个古老的蛋白质家族，其氨基酸序列从苔藓到被子植物高度保守。OsB 12 D1是OsB 12 Ds的主要转录本之一，主要在萌发种子和根中表达。生物信息学分析表明OsB 12 D1是一个耐缺氧或耐淹相关基因。RT-PCR结果表明，种子萌发和幼苗早期生长过程中，淹水可明显诱导OsB 12 D1在胚芽鞘或根中表达。OsB 12 D1过表达的水稻种子在8厘米深的水中可以伸出胚根，与野生型相比进一步表现出显着的耐淹性。此外，这种耐受性不受赤霉素生物合成抑制剂多效唑的影响。OsB 12 D1通过亚细胞定位分析被确定在细胞中，并可能通过介导铁和氧的可用性在淹水条件下增强电子传递。本研究表明OsB 12 D1是一个很有希望的基因，可以帮助提高水稻在农业实践中的成苗率，特别是直播。
B12D belongs to a function unknown subgroup of the Balem (Barley aleurone and embryo) proteins. In our previous work on rice seed germination, we identified a B12D-like protein encoded by LOC_Os7g41350 (named OsB12D1). OsB12D1 pertains to an ancient protein family with an amino acid sequence highly conserved from moss to angiosperms. Among the six OsB12Ds, OsB12D1 is one of the major transcripts and is primarily expressed in germinating seed and root. Bioinformatics analyses indicated that OsB12D1 is an anoxic or submergence resistance-related gene. RT-PCR results showed OsB12D1 is induced remarkably in the coleoptiles or roots by flooding during seed germination and early seedling growth. The OsB12D1-overexpressed rice seeds could protrude radicles in 8 cm deep water, further exhibiting significant flooding tolerance compared to the wild type. Moreover, this tolerance was not affected by the gibberellin biosynthesis inhibitor paclobutrazol. OsB12D1 was identified in the mitochondrion by subcellular localization analysis and possibly enhances electron transport through mediating Fe and oxygen availability under flooded conditions. This work indicated that OsB12D1 is a promising gene that can help to enhance rice seedling establishment in farming practices, especially for direct seeding.
登录
查看更多内容
影响因子：
7.4
作者：
Agrawal, GK;Yamazaki, M;Hirochika, H
通讯作者：
Hirochika, H
定量蛋白质组学揭示蛋白质磷酸化在水稻胚发芽早期的作用
影响因子：
4.4
作者：
Han, Chao;Yang, Pingfang;Komatsu, Setsuko
通讯作者：
Komatsu, Setsuko
影响因子：
7.4
作者：
Howell, Katharine A.;Narsai, Reena;Whelan, James
通讯作者：
Whelan, James
影响因子：
3.4
作者：
He, Dongli;Han, Chao;Yang, Pingfang
通讯作者：
Yang, Pingfang
影响因子：
5.1
作者：
Howell, KA;Millar, AH;Whelan, J
通讯作者：
Whelan, J