Analysis of Melanin Structure and Biochemical Composition in Conjunctival Melanocytic Lesions Using Pump–Probe Microscopy
Analysis of Melanin Structure and Biochemical Composition in Conjunctival Melanocytic Lesions Using Pump–Probe Microscopy
复制标题
使用泵探针显微镜分析结膜黑素细胞病变中的黑色素结构和生化成分
DOI：
10.1167/tvst.8.3.33
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发表时间：
2019
影响因子：
3
通讯作者：
Warren, Warren S.
中科院分区：
文献类型：
--
作者：
Robles, Francisco E.;Deb, Sanghamitra;Vajzovic, Lejla;Vora, Gargi K.;Mruthyunjaya, Prithvi;Warren, Warren S.
目的：我们利用泵浦探测显微镜分析结膜黑素细胞病变中的黑色素结构和生化成分，以评估这种方法在黑色素瘤诊断中的潜力。方法：泵浦探测显微镜询问吸收分子的瞬时激发态光动力学性质，从而产生具有亚细胞空间分辨率的高度特异性的分子信息。应用这种方法分析了39例黑素细胞结膜病变的未染色薄层活检标本中的黑色素。利用主成分分析的几何表示和数学形态学的原理，对组织病理标本中黑色素的生化组成和结构的定量特征进行了评估。结果：结膜黑色素瘤与无严重异型性的原发性获得性结膜黑变病(PAM)相比，表现出更高的生化异质性和不同的整体生化成分。与其他类型的PAM相比，具有严重异型性的PAM的分子特征更接近于黑色素瘤。随着病变诊断的恶化，组织中的色素组织变得更加混乱，但色素痣在生化和结构上比其他病变更不一致。对于不同类型的黑素细胞病变，尤其是无严重异型性和黑色素瘤的PAM，获得了相对较高的敏感度(SE)和特异度(SP)(SE=%；SP=87%)。结论：泵浦-探针显微镜是一种强大的工具，可以识别不同类型结膜黑素细胞病变之间的定量表型差异。翻译相关性：本研究进一步验证了泵-探头显微镜作为结膜黑素细胞病变组织病理学诊断的潜在辅助手段的使用。
Purpose: We analyze melanin structure and biochemical composition in conjunctival melanocytic lesions using pump-probe microscopy to assess the potential for this method to assist in melanoma diagnosis.Methods: Pump–probe microscopy interrogates transient excited-state photodynamic properties of absorbing molecules, which yields highly specific molecular information with subcellular spatial resolution. This method is applied to analyze melanin in 39 unstained, thin biopsy specimens of melanocytic conjunctival lesions. Quantitative features of the biochemical composition and structure of melanin in histopathologic specimens are assessed using a geometric representation of principal component analysis (PCA) and principles of mathematical morphology. Diagnostic power is determined using a feature selection algorithm combined with cross validation.Results: Conjunctival melanomas show higher biochemical heterogeneity and different overall biochemical composition than primary acquired melanosis of the conjunctiva (PAM) without severe atypia. The molecular signatures of PAMs with severe atypia more closely resemble melanomas than other types of PAMs. Pigment organization in the tissue becomes more disorganized as diagnosis of the lesions worsen, but nevi are more inconsistent biochemically and structurally than other lesions. Relatively high sensitivity (SE) and specificity (SP) is achieved for differentiating between various melanocytic lesions, particularly PAMs without severe atypia and melanomas (SE= 89%; SP= 87%).Conclusions: Pump–probe microscopy is a powerful tool that can identify quantitative, phenotypic differences between various types of conjunctival melanocytic lesions.Translational Relevance: This study further validates the use of pump–probe microscopy as a potential diagnostic aid for histopathologic evaluation of conjunctival melanocytic lesions.
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结膜的痣和黑色素瘤*†
影响因子：
4.1
作者：
B. Jay
通讯作者：
B. Jay
影响因子：
1.7
作者：
Glusac, Earl J.
通讯作者：
Glusac, Earl J.
DOI：
10.1016/j.jtos.2015.02.001
发表时间：
2015-07
期刊：
The ocular surface
影响因子：
--
作者：
Nanji AA;Sayyad FE;Galor A;Dubovy S;Karp CL
通讯作者：
Karp CL
影响因子：
3.5
作者：
Fu, Dan;Ye, Tong;Warren, S. Warren
通讯作者：
Warren, S. Warren
影响因子：
3.8
作者：
Robles FE;Wilson JW;Fischer MC;Warren WS
通讯作者：
Warren WS