Staring at the cold sun: blue light regulation is distributed within the genus Acinetobacter.
Staring at the cold sun: blue light regulation is distributed within the genus Acinetobacter.
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DOI：
10.1371/journal.pone.0055059
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Mussi MA
中科院分区：
文献类型：
--
作者：
Golic A;Vaneechoutte M;Nemec A;Viale AM;Actis LA;Mussi MA
我们之前的研究表明，机会性医院病原体鲍曼不动杆菌能够通过BlsA（一种BLUF （Blue-Light-sensing Using FAD）结构域光感受器蛋白）感知光并对光做出反应。在这里，我们扩展了我们以前的研究，表明光调节不仅限于鲍曼不动杆菌，而且在不动杆菌属中相当广泛。首先，我们发现蓝光可以调节该属许多物种的运动和生物膜形成，包括不动杆菌calcoaceticus-A的成员。baumannii复杂。在许多这些物种中，蓝光在24°C下是指导运动或不运动的关键因素，而在鲍曼不动虫中，光既抑制运动又抑制生物膜的形成。我们还发现，在非a条件下，光调节运动不仅发生在24°C，也发生在37°C。鲍曼不动杆菌仅在24°C下表现光调节。其次，我们发现贝氏不动杆菌（菌株ADP1） blf光感受器在体内可以在功能上替代鲍曼不动杆菌17978 BlsA蛋白，并且在24°C下，这两种微生物之间导致生物膜形成的途径是反向调节的。最后，我们发现在所有不动杆菌测序基因组中存在编码含有bluf蛋白的预测基因，即使它们之间的拷贝数是可变的。系统发育分析表明，该属成员中存在所有BLUF结构域的共同起源，并且可以区分来自不同物种的BLUF同源物组合在一起的分化良好的簇，这种情况对ACB复合体的成员尤其明显。尽管在不动杆菌属成员中观察到的这些含有BLUF结构域的蛋白在光调节中发挥作用是一种可能的情况，鉴于我们在鲍曼不动杆菌和巴利不动杆菌中的发现，进一步的研究将有助于证实这种可能性。
We previously showed that the opportunistic nosocomial pathogen Acinetobacter baumannii is able to sense and respond to light via BlsA, a BLUF (Blue-Light-sensing Using FAD)-domain photoreceptor protein. Here, we extend our previous studies showing that light regulation is not restricted to A. baumannii, but rather widespread within the genus Acinetobacter. First, we found that blue light modulates motility and biofilm formation in many species of the genus, including members of the Acinetobacter calcoaceticus-A. baumannii complex. In many of these species blue light acts as a key factor guiding the decision between motility or sessility at 24°C, whereas in A. baumannii, light inhibits both motility and biofilm formation. We also show that light regulation of motility occurred not only at 24°C but also at 37°C in non-A. baumannii species, contrasting the situation of A. baumannii which only shows photoregulation at 24°C. Second, we show that Acinetobacter baylyi (strain ADP1) BLUF-photoreceptors can functionally replace in vivo the A. baumannii 17978 BlsA protein and that the pathways leading to biofilm formation are inversely regulated at 24°C between these two microorganisms. Finally, we found the presence of predicted genes coding BLUF-containing proteins in all Acinetobacter sequenced genomes, even though the copy number is variable among them. Phylogenetic analysis suggests a common origin for all BLUF domains present in members of this genus, and could distinguish well-differentiated clusters that group together BLUF homologs from different species, a situation particularly clear for members of the ACB complex. Despite a role played by these BLUF domain-containing proteins in the photoregulation observed in the members of the genus Acinetobacter is a likely scenario given our findings in A. baumannii and A. baylyi, further research will contribute to confirm this possibility.
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DOI：
10.1099/ijs.0.02486-0
发表时间：
2003-07-01
影响因子：
2.8
作者：
Carr, EL;Kämpfer, P;Seviour, RJ
通讯作者：
Seviour, RJ
影响因子：
3
作者：
Anandham, Rangasamy;Weon, Hang-Yeon;Kwon, Soon-Wo
通讯作者：
Kwon, Soon-Wo
DOI：
10.1099/ijs.0.02631-0
发表时间：
2003-09-01
影响因子：
2.8
作者：
Nemec, A;Dijkshoorn, L;Vaneechoutte, M
通讯作者：
Vaneechoutte, M
影响因子：
3.7
作者：
Diancourt L;Passet V;Nemec A;Dijkshoorn L;Brisse S
通讯作者：
Brisse S
影响因子：
13.8
作者：
Gomelsky, M;Klug, G
通讯作者：
Klug, G