A Perilipin Affects Lipid Droplet Homeostasis and Aerial Hyphal Growth, but Has Only Small Effects on Virulence in the Insect Pathogenic Fungus Beauveria bassiana.
A Perilipin Affects Lipid Droplet Homeostasis and Aerial Hyphal Growth, but Has Only Small Effects on Virulence in the Insect Pathogenic Fungus Beauveria bassiana.
复制标题
DOI：
10.3390/jof8060634
复制
发表时间：
2022-06-15
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
脂质的同化、储存和周转影响包括真菌在内的许多微生物病原体的生长、发育和毒力。Perilipins是与脂滴(LDs)相关的蛋白质，介导它们的组装和周转。在这里，我们对球孢白僵菌(BbPlin1)Perilipin进行了表征。BbPlin1在最低限度的培养基中的表达高于在丰富的培养基中的表达，并且使用BbPlin1：：EGFP融合蛋白，该蛋白被证明共定位于LDS，在昆虫(Galleria Mellonella)宿主内的感染和增殖过程中高表达，在包括分生孢子在内的身体上的真菌生长期间显著降低至几乎不表达，但一旦将BbPlin1放入允许萌发和生长的含有营养的培养基中，分生孢子中BbPlin1的产生就恢复了。对目标基因缺失菌株(ΔBbPlin1)的鉴定表明，细胞内LD含量显著(>；30%)减少，促进了气生菌丝的生长，毒力略有下降，对营养生长和胁迫反应几乎没有影响。然而，在ΔBbPlin1株中，互补的LD相关Caleosin基因BbCal1的表达在营养不良的条件下被增强，尽管在ΔBbCal1株中没有看到BbPlin1的表达变化，并且在ΔBbCal1株中的BbPlin1的表达没有改变细胞中的LD模式。转录组和RT-PCR分析表明，在BbPlin1缺失突变体中，与脂代谢相关的基因表达增加，包括三酰甘油脂肪酶3、Enoyl-CoA异构酶和二酰甘油-O-乙酰基转移酶。脂谱分析证实，与野生型菌株相比，BbPlin1基因的缺失显著降低了细胞中三酰甘油、二酰甘油和磷脂酰乙醇胺的含量。这些结果表明，球孢杆菌Perilipin参与了调节脂质平衡、真菌气生菌丝生长和毒力的过程，揭示了从宿主体内养分利用期间的高表达到感染过程中身体表面生长期间的低表达的关键循环。
Lipid assimilation, storage, and turnover impact growth, development, and virulence in many microbial pathogens including fungi. Perilipins are proteins associated with lipid droplets (LDs) that mediate their assembly and turnover. Here, we characterized the Beauveria bassiana (BbPlin1) perilipin. BbPlin1 expression was higher in minimal media than in rich media, and, using a BbPlin1::eGFP fusion protein, the protein was shown to be co–localized to LDs, with the high expression seen during infection and proliferation within the insect (Galleria mellonella) host that dramatically decreased to almost no expression during fungal outgrowth on cadavers including in conidia, but that BbPlin1 production resumed in the conidia once placed in nutrient–containing media allowing for germination and growth. Characterization of a targeted gene deletion strain (ΔBbPlin1) revealed a dramatic (>30%) reduction in cellular LD content, promotion of aerial hyphal growth, and a small decrease in virulence, with little to no effects on vegetative growth and stress responses. However, in the ΔBbPlin1 strain, expression of the complementary LD–associated caleosin gene, BbCal1, was enhanced under nutrient–poor conditions, although no changes in BbPlin1 expression were seen in a ΔBbCal1 strain and the expression of BbPlin1 in the ΔBbCal1 strain did not change LD patterns in cells. Transcriptome and RT–PCR analyses indicated increased expression of lipid metabolism–related genes, including triacylglyercol lipase 3, enoyl–CoA isomerase, and diacylglycerol–O–acetyl transferase in the BbPlin1 deletion mutant. Lipid profile analyses confirmed that the loss of BbPlin1 significantly reduced the cellular levels of contents of triacylglycerol, diacylglycerol, and phosphatidylethanolamine as compared to the wild–type strain. These results demonstrate the involvement of the B. bassiana perilipin in mediating lipid homeostasis, fungal aerial hyphal growth, and virulence, revealing critical cycling from high expression during nutrient utilization within host cadavers to low expression during growth on the surface of the cadaver during the infection process.
登录
查看更多内容
影响因子：
11.1
作者：
Krahmer, Natalie;Farese, Robert V., Jr.;Walther, Tobias C.
通讯作者：
Walther, Tobias C.
影响因子：
4.5
作者：
Itabe H;Yamaguchi T;Nimura S;Sasabe N
通讯作者：
Sasabe N
DOI：
10.1016/j.bbalip.2009.04.002
发表时间：
2009-06
影响因子：
4.8
作者：
Bickel, Perry E.;Tansey, John T.;Welte, Michael A.
通讯作者：
Welte, Michael A.
影响因子：
4
作者：
Guo, Yi;Cordes, Kimberly R.;Walther, Tobias C.
通讯作者：
Walther, Tobias C.
影响因子：
29
作者：
Beller, Mathias;Bulankina, Anna V.;Kuehnlein, Ronald P.
通讯作者：
Kuehnlein, Ronald P.