High prevalence of Beijing and EAI4-VNM genotypes among M. tuberculosis isolates in northern Vietnam: sampling effect, rural and urban disparities.
High prevalence of Beijing and EAI4-VNM genotypes among M. tuberculosis isolates in northern Vietnam: sampling effect, rural and urban disparities.
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DOI：
10.1371/journal.pone.0045553
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Dang DA
中科院分区：
文献类型：
--
作者：
Nguyen VA;Choisy M;Nguyen DH;Tran TH;Pham KL;Thi Dinh PT;Philippe J;Nguyen TS;Ho ML;Van Tran S;Bañuls AL;Dang DA
221株M.结核病的样本来自越南北方平原的医院和普通人群，该平原是越南人口最多的地区之一。基因型组成和多样性进行了表征，我们调查了它们如何受到采样（医院与普通人群）的影响，校正了潜在的混杂效应（患者的位置，年龄和性别）。以Spoligotyping和12 MIRU-VNTR分型为一线。然后用15套MIRU-VNTR标准品对聚类的21株分离株进行MIRU-VNTR分型。结果表明，该地区有8个支系和13个亚支系。北京地区和EAI地区是最主要的家系，分别占38.5%和38.5%。其他出现小比例H（1.4%）、LAM（1.8%）、T（8.1%）、X（0.9%）、MANU（2.3%）和零（0.4%）。医院样本的聚集率（城市为17.9%，农村为19.2%）高于人群样本（0%）。EAI中典型的越南EAI 4-VNM亚系占EAI菌株的67%，且与年龄较大有关。北京基因型与年轻的城市人群相关，具有高聚集率的特点。这些特征有力地表明，北京菌株正在侵入人群，取代当地的EAI-VNM 4，从而预测在缺乏更有效的控制策略的情况下，未来的结核病形势将更加严重。
A total of 221 isolates of M. tuberculosis were sampled from hospitals and the general population in the northern plain of Vietnam, one of the most populated region of the country. Genotypic composition and diversity were characterized, and we investigated how they are affected by sampling (hospital vs. general population), correcting for potential confounding effects (location, age and gender of the patients). Spoligotyping and 12 MIRU-VNTR typing were used as first line. Then 15 MIRU-VNTR standard set was used, making 21 MIRU-VNTR typing for the clustered isolates. Result showed that 8 lineages and 13 sub-lineages were circulating in the region. The most predominant lineages were Beijing (38.5%) and EAI (38.5%). Others appeared with small proportions H (1.4%), LAM (1.8%), T (8.1%), X (0.9%), MANU (2.3%), and Zero (0.4%). Higher clustering rate was found in the hospital samples (17.9% in urban and 19.2% in rural areas) compared to the population ones (0%). The typical Vietnamese EAI4-VNM sub-lineage of EAI lineage accounted for 67% of EAI strains and was associated with older ages. Beijing genotypes were associated with younger, urban population and were characterized by high clustering rates. These characteristics strongly suggest that Beijing strains are invading the population, replacing the local EAI-VNM4, thus predicting a more serious tuberculosis situation in the future in the absence of more effective control strategies.
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