OligoCalc: an online oligonucleotide properties calculator.

OligoCalc: an online oligonucleotide properties calculator.
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DOI:
10.1093/nar/gkm234
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发表时间:
2007-07
影响因子:
14.9
通讯作者:
Kibbe WA
Kibbe WA
中科院分区:
生物学2区
文献类型:
--
作者:
Kibbe WA

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我们开发了 OligoCalc 作为一种可通过网络访问、基于客户端的计算引擎,用于报告 DNA 和 RNA 单链和双链特性,包括分子量、溶液浓度、解链温度、估计吸光度系数、分子间自互补性估计和分子内发夹环形成。 OligoCalc 具有熟悉的“计算器”外观和感觉,使其易于理解和使用。 OligoCalc 结合了三种计算寡核苷酸熔解温度的常用方法,包括熔解温度的最近邻热力学模型。自 1997 年首次上线以来,OligoCalc 的访问量已超过 900 万次,来自近 20 万个不同的主机(不包括搜索引擎)。 OligoCalc 可在 http://basic.northwestern.edu/biotools/OligoCalc.html 上获取,该链接还提供完整源代码、使用模式和统计数据的链接。
We developed OligoCalc as a web-accessible, client-based computational engine for reporting DNA and RNA single-stranded and double-stranded properties, including molecular weight, solution concentration, melting temperature, estimated absorbance coefficients, inter-molecular self-complementarity estimation and intra-molecular hairpin loop formation. OligoCalc has a familiar ‘calculator’ look and feel, making it readily understandable and usable. OligoCalc incorporates three common methods for calculating oligonucleotide-melting temperatures, including a nearest-neighbor thermodynamic model for melting temperature. Since it first came online in 1997, there have been more than 900 000 accesses of OligoCalc from nearly 200 000 distinct hosts, excluding search engines. OligoCalc is available at http://basic.northwestern.edu/biotools/OligoCalc.html, with links to the full source code, usage patterns and statistics at that link as well.
DOI: 10.1093/nar/gkh435
发表时间: 2004-07-01
影响因子: 14.9
作者:
McGinnis, S;Madden, TL
通讯作者: Madden, TL
DOI: 10.1093/nar/gki379
发表时间: 2005-07-01
影响因子: 14.9
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DOI: 10.1073/pnas.83.11.3746
发表时间: 1986-06-01
影响因子: 11.1
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通讯作者: MARKY, LA
DOI: 10.1073/pnas.95.4.1460
发表时间: 1998-02-17
影响因子: 11.1
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DOI: 10.1093/nar/gkg595
发表时间: 2003-07-01
影响因子: 14.9
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