A2-type cyclin is required for the asymmetric entry division in rice stomatal development.
A2-type cyclin is required for the asymmetric entry division in rice stomatal development.
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水稻气孔发育中的不对称进入分裂需要 A2 型细胞周期蛋白
DOI：
10.1093/jxb/ery158
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发表时间：
2018-06-27
影响因子：
6.9
通讯作者：
Le J
中科院分区：
文献类型：
--
作者：
Qu X;Yan M;Zou J;Jiang M;Yang K;Le J
水稻基因组包含一个编码A2型细胞周期蛋白的基因OsCYCA 2;1，A2型细胞周期蛋白是一种进化上保守的蛋白质，是细胞不对称分裂产生气孔谱系细胞所必需的。在水稻和其他主要禾谷类作物中，气孔排列成平行于叶片长轴的线性文件。每个气孔单位由两个哑铃形的保卫细胞和两个附属细胞组成。这些形态和发育特征使气孔能够更有效地响应环境变化。细胞周期蛋白依赖性激酶（CDK）及其细胞周期蛋白伴侣在多细胞生物体发育过程中协调细胞增殖和分化。与动物相比，植物有更多类型和成员的细胞周期蛋白。在拟南芥中，4个A2型细胞周期蛋白（CYCA 2s）在调节CDKB 1活性方面发挥冗余作用，以促进气孔起始的不对称分裂和保卫母细胞（GMCs）的对称分裂。在这项研究中，我们研究了水稻中单个A2型细胞周期蛋白OsCYCA 2;1，以及单个B1型CDK OsCDKB 1;1的功能。跨种互补试验表明，OsCYCA 2;1和OsCDKB 1;1不仅能互补拟南芥cyca 2和cdkb 1突变体的气孔缺陷表型，还能抑制DNA内复制和细胞膨大。在OsCYCA 2;1-RNAi转基因水稻的气孔细胞档案中，建立气孔谱系的早期不对称分裂通常缺失，导致气孔产量显著降低。然而，在OsCYCA 2;1-RNAi和OsCDKB 1;1-RNAi转基因水稻中，GMC分裂没有如预期的那样被破坏。我们的研究结果表明，水稻A2型细胞周期蛋白的功能和行为保守，但分歧，这可能与水稻和拟南芥之间的不同气孔发育途径。
The rice genome contains a single gene OsCYCA2;1 encoding A2-type cyclin, which is an evolutionarily conserved protein required for asymmetric cell divisions to produce stomatal lineage cells. In rice, and other major cereal grass crops, stomata are arranged in linear files parallel to the long growth axis of leaves. Each stomatal unit comprises two dumbbell-shaped guard cells flanked by two subsidiary cells. These morphological and developmental characteristics enable grass stomata to respond to environmental changes more efficiently. Cyclin-dependent kinases (CDKs) and their cyclin partners co-ordinate cell proliferation and differentiation during the development of multicellular organisms. In contrast to animals, plants have many more types and members of cyclins. In Arabidopsis, four A2-type cyclins (CYCA2s) function redundantly in regulating CDKB1 activity to promote the asymmetric division for stomatal initiation and the symmetric division of guard mother cells (GMCs). In this study, we examine the function of the single A2-type cyclin in rice, OsCYCA2;1, as well the single B1-type CDK, OsCDKB1;1. Cross-species complementation tests demonstrated that OsCYCA2;1 and OsCDKB1;1 could complement the defective stomatal phenotypes of Arabidopsis cyca2 and cdkb1 mutants, but also could suppress DNA endoduplication and cell enlargement. The early asymmetric divisions that establish the stomatal lineages are often missing within the stomatal cell files of OsCYCA2;1-RNAi rice transgenic lines, leading to a significantly reduced stomatal production. However, GMC divisions are not disrupted either in OsCYCA2;1-RNAi or in OsCDKB1;1-RNAi rice transgenic lines as expected. Our results demonstrate a conserved but diverged function and behavior of rice A2-type cyclins, which might be associated with the distinct stomatal development pathways between rice and Arabidopsis.
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