A novel bacterial pathogen of Biomphalaria glabrata: a potential weapon for schistosomiasis control?
A novel bacterial pathogen of Biomphalaria glabrata: a potential weapon for schistosomiasis control?
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DOI：
10.1371/journal.pntd.0003489
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发表时间：
2015-02
影响因子：
3.8
通讯作者：
Gourbal B
中科院分区：
文献类型：
--
作者：
Duval D;Galinier R;Mouahid G;Toulza E;Allienne JF;Portela J;Calvayrac C;Rognon A;Arancibia N;Mitta G;Théron A;Gourbal B
血吸虫病是仅次于疟疾的第二大热带寄生虫病。世界卫生组织建议并支持在10年内实现消灭血吸虫病目标的各种研究策略和治疗方案。其中一种办法是在地方病流行地区控制蜗牛病媒。以往的现场研究表明，竞争对手或捕食者的引入可以减少蜗牛的数量，但没有进行系统的调查，以确定蜗牛微生物病原体和评价其杀螺效果。在经历高死亡率的光滑双脐螺种群中，在螺体上可见白色结节。从这些结节中分离出感染性病原体。其中仅发现一种细菌，经鉴定为类芽孢杆菌属一新种，命名为光滑类芽孢杆菌（Paenibacillusglabratella），并经16 S和Rpob DNA分析与蜂房类芽孢杆菌（P. alvei）亲缘关系较近。组织学检查显示广泛的细菌浸润，导致整体组织解体。健康的蜗牛暴露于感染类芽孢杆菌的蜗牛会导致大量死亡。此外，受感染的蜗牛产下的卵也被感染，孵化减少，但对产卵没有明显影响。胚胎致死率与鸡蛋中存在的致病菌有关。这是一个新的类芽孢杆菌菌株，Ca。光滑类芽孢杆菌是一种蜗牛病原微生物。由于这种菌株影响成虫和胚胎阶段，并导致显着的死亡率，它可能有希望作为一种生物控制剂，以限制血吸虫病的传播领域。本文报道了一种新的淡水蜗牛病原菌-光滑双脐螺的分离和鉴定。遗传分析显示，该物种以前没有被描述过，可以归类为类芽孢杆菌属。这些细菌侵入大多数蜗牛组织并增殖，造成大规模的致命性。此外，细菌感染可以垂直和水平传播给其他蜗牛，导致它们在30天内死亡。这一发现具有潜在的重要性，因为B。作为中间宿主，光滑虫在传播血吸虫病这一人类第二大寄生虫病方面发挥重要作用。世界卫生组织在十年内消灭血吸虫病的目标鼓励发展多种方法来对付这种疾病，其中之一是病媒种群控制。这种新的细菌菌株显然可能是这种策略的潜在代理人。
Schistosomiasis is the second-most widespread tropical parasitic disease after malaria. Various research strategies and treatment programs for achieving the objective of eradicating schistosomiasis within a decade have been recommended and supported by the World Health Organization. One of these approaches is based on the control of snail vectors in endemic areas. Previous field studies have shown that competitor or predator introduction can reduce snail numbers, but no systematic investigation has ever been conducted to identify snail microbial pathogens and evaluate their molluscicidal effects. In populations of Biomphalaria glabrata snails experiencing high mortalities, white nodules were visible on snail bodies. Infectious agents were isolated from such nodules. Only one type of bacteria, identified as a new species of Paenibacillus named Candidatus Paenibacillus glabratella, was found, and was shown to be closely related to P. alvei through 16S and Rpob DNA analysis. Histopathological examination showed extensive bacterial infiltration leading to overall tissue disorganization. Exposure of healthy snails to Paenibacillus-infected snails caused massive mortality. Moreover, eggs laid by infected snails were also infected, decreasing hatching but without apparent effects on spawning. Embryonic lethality was correlated with the presence of pathogenic bacteria in eggs. This is the first account of a novel Paenibacillus strain, Ca. Paenibacillus glabratella, as a snail microbial pathogen. Since this strain affects both adult and embryonic stages and causes significant mortality, it may hold promise as a biocontrol agent to limit schistosomiasis transmission in the field. The present paper reports the isolation and the characterization of a new microbial pathogen of the freshwater snail, Biomphalaria glabrata. Genetic analyses revealed that the species has not been previously described and could be classified into the Paenibacillus genus. These bacteria invade most snail tissues and proliferate, causing massive lethality. Moreover, the bacterial infection can be transmitted both vertically and horizontally to other snails, causing their death in 30 days. This discovery is potentially important because B. glabrata, as an intermediate host, plays an important role in transmitting schistosomiasis, the second-most widespread human parasitic disease. The World Health Organization’s objective of schistosomiasis eradication in a decade encourages the development of multiple approaches for countering the disease, one of which is vector population control. This new bacterial strain clearly could be a potential agent for such a strategy.
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影响因子：
2.2
作者：
Contreras, J.;Mendoza, J. E.;Bielza, P.
通讯作者：
Bielza, P.
影响因子：
2.4
作者：
Bouchut, A.;Coustau, C.;Mitta, G.
通讯作者：
Mitta, G.
影响因子：
2.1
作者：
CREWS, AE;YOSHINO, TP
通讯作者：
YOSHINO, TP
影响因子：
4.4
作者：
de Oliveira, EJ;Rabinovitch, L;Zahner, V
通讯作者：
Zahner, V
影响因子：
3.8
作者：
Bravo, Alejandra;Likitvivatanavong, Supaporn;Gill, Sarjeet S.;Soberon, Mario
通讯作者：
Soberon, Mario