Directed differentiation of human-induced pluripotent stem cells generates active motor neurons.
Directed differentiation of human-induced pluripotent stem cells generates active motor neurons.
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DOI：
10.1002/stem.31
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发表时间：
2009-04
期刊：
影响因子：
5.2
通讯作者：
Lowry, William E.
中科院分区：
文献类型：
--
作者：
Karumbayaram, Saravanan;Novitch, Bennett G.;Patterson, Michaela;Umbach, Joy A.;Richter, Laura;Lindgren, Anne;Conway, Anne E.;Clark, Amander T.;Goldman, Steve A.;Plath, Kathrin;Wiedau-Pazos, Martina;Kornblum, Harley I.;Lowry, William E.
人类诱导多能干 (iPS) 细胞定向分化为功能性有丝分裂后神经元表型的潜力尚不清楚。遵循被证明可有效从人胚胎干细胞 (hESC) 生成运动神经元的方法，我们发现一旦指定为神经谱系，人 iPS 细胞就可以分化形成运动神经元，其效率与 hESC 相似。人类 iPS 衍生细胞似乎遵循与运动神经元形成相关的正常发育进程，并具有典型的电生理特性。这是首次证明人类 iPS 衍生细胞能够产生电活性运动神经元。这些发现证明了在再生医学应用和运动神经元疾病体外建模中使用 iPS 衍生的运动神经元祖细胞和运动神经元的可行性。
The potential for directed differentiation of human induced pluripotent stem (iPS) cells to functional post-mitotic neuronal phenotypes is unknown. Following methods shown to be effective at generating motor neurons from human embryonic stem cells (hESCs), we found that once specified to a neural lineage, human iPS cells could be differentiated to form motor neurons with a similar efficiency as hESCs. Human iPS-derived cells appeared to follow a normal developmental progression associated with motor neuron formation and possessed prototypical electrophysiological properties. This is the first demonstration that human iPS-derived cells are able to generate electrically active motor neurons. These findings demonstrate the feasibility of using iPS-derived motor neuron progenitors and motor neurons in regenerative medicine applications and in vitro modeling of motor neuron diseases.
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影响因子：
64.8
作者：
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通讯作者：
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影响因子：
16.2
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作者：
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通讯作者：
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