Biomechanical remodelling of obstructed guinea pig jejunum.
Biomechanical remodelling of obstructed guinea pig jejunum.
复制标题
DOI：
10.1016/j.jbiomech.2010.01.018
复制
发表时间：
2010-05-07
影响因子：
2.4
通讯作者：
Gregersen, Hans
中科院分区：
文献类型：
--
作者：
Zhao, Jingbo;Liao, Donghua;Yang, Jian;Gregersen, Hans
需要形态学和生物力学重建的数据来了解肠梗阻背后的机制。本研究测定了部分梗阻对豚鼠小肠的零应力状态力学性能和组织形态学性能的影响。在豚鼠空肠中段手术造成部分梗阻和假手术。动物分别存活2、4、7和14天。年龄匹配的未手术豚鼠作为正常对照。梗阻部位近端的节段用于组织学分析、无负荷状态和零应力状态数据以及扩张试验。将用于扩张的节段浸入器官浴中并膨胀至10 cm H2O。使用带有CCD相机的显微镜监测充盈期间的外径变化。根据直径、压力和零应力状态数据计算周向应力和应变。梗阻7d后，开放角和残余应变绝对值减小（P<0.01和P<0.001），而管壁厚度、管壁横截面积和管壁刚度增加（P<0.05，P<0.01）。组织学上，肠梗阻后肌层和粘膜下层，尤其是周围肌层增厚。张开角和残余应变主要取决于肌层厚度，而壁刚度主要取决于粘膜下层厚度。总之，小肠的组织形态学和生物力学特性（首次参考零应力状态）以时间依赖性方式在梗阻部位近端重塑。
Data on morphological and biomechanical remodeling are needed to understand the mechanisms behind intestinal obstruction. The effect of partial obstruction on mechanical properties with reference to the zero-stress state and on the histomorphological properties of the guinea pig small intestine was determined in this study. Partial obstruction and sham operation were surgically created in mid-jejunum of guinea pigs. The animals survived 2, 4, 7, and 14 days respectively. The age-matched guinea pigs that were not operated served as normal controls. The segment proximal to the obstruction site was used for histological analysis, no-load state and zero-stress state data, and distension test. The segment for distension was immersed in an organ bath and inflated to 10 cmH20. The outer diameter change during the inflation was monitored using a microscope with CCD camera. Circumferential stresses and strains were computed from the diameter, pressure and the zero-stress state data. The opening angle and absolute value of residual strain decreased (P<0.01 and P<0.001) whereas the wall thickness, wall cross-sectional area, and the wall stiffness increased after 7 days obstruction (P<0.05, P<0.01). Histologically, the muscle and submucosa layers, especially the circumferential muscle layer increased in thickness after obstruction. The opening angle and residual strain mainly depended on the thickness of the muscle layer whereas the wall stiffness mainly depended on the thickness of the submucosa layer. In conclusion, the histomorphological and biomechanical properties of small intestine (referenced for the first time to the zero-stress state) remodel proximal to the obstruction site in a time-dependent manner.
登录
查看更多内容
影响因子：
3.6
作者：
GABELLA, G
通讯作者：
GABELLA, G
影响因子：
2.4
作者：
Lu, X;Zhao, JB;Gregersen, H
通讯作者：
Gregersen, H
影响因子：
3.5
作者：
Chen J;Chen H;Sanders KM;Perrino BA
通讯作者：
Perrino BA
影响因子：
7.7
作者：
LIU, SQ;FUNG, YC
通讯作者：
FUNG, YC
影响因子：
3.1
作者：
Storkholm, JH;Villadsen, GE;Gregersen, H
通讯作者：
Gregersen, H