Substitution Mapping and Allelic Variations of the Domestication Genes from O. rufipogon and O. nivara.
Substitution Mapping and Allelic Variations of the Domestication Genes from O. rufipogon and O. nivara.
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DOI：
10.1186/s12284-023-00655-y
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发表时间：
2023-09-05
期刊：
影响因子：
5.5
通讯作者：
Fu, Xuelin
中科院分区：
文献类型：
--
作者：
Wang, Zhangqiang;Guo, Zisheng;Zou, Tuo;Zhang, Zhe;Zhang, Jianan;He, Ping;Song, Ruifeng;Liu, Ziqiang;Zhu, Haitao;Zhang, Guiquan;Fu, Xuelin
关键词：
从野生稻到栽培稻的驯化是一个重要的里程碑，涉及到许多特定性状的变化和遗传系统的变异。在aa基因组野生稻品种中，O. rufipogon和O. nivara具有许多有利基因，被认为是O. sativa的祖先。本研究以优质籼稻品种HJX74为背景，以O. rufipogon和O. nivara为供体，培育了单片段代换系。在SSSLs群体中，鉴定出分蘖角、展穗、芒、落粒、红果皮等5个驯化性状的11个基因，并通过置换作图将其定位在5条染色体上。通过与已知基因的序列比对，发现了7个基因的等位基因变异，即TA7-RUF与PROG1等位，TA8-RUF与TIG1等位，SPR4-NIV与OsLG1等位，AN4-RUF与An-1等位，SH4- niv与SH4等位，RC7-RUF和RC7-NIV均与Rc等位。同时，TA11-NIV、SPR3-NIV、AN3-NIV和AN4-NIV 4个基因被认为是在这些sssl中发现的新基因，因为在这些基因的染色体位置没有发现与驯化性状相关的已知基因。结果表明，SSSLs将成为野生稻品种基因挖掘和利用的宝贵种质资源，为进一步分析新的驯化基因奠定基础，更好地了解驯化过程中调控性状变异的遗传基础。在线版本包含补充材料，可在10.1186/s12284-023-00655-y获得。
Domestication from wild rice species to cultivated rice is a key milestone, which involved changes of many specific traits and the variations of the genetic systems. Among the AA-genome wild rice species, O. rufipogon and O. nivara, have many favorable genes and thought to be progenitors of O. sativa. In the present study, by using O. rufipogon and O. nivara as donors, the single segment substitution lines (SSSLs) have been developed in the background of the elite indica cultivar, HJX74. In the SSSLs population, 11 genes for 5 domestication traits, including tiller angle, spreading panicle, awn, seed shattering, and red pericarp, were identified and mapped on 5 chromosomes through substitution mapping. Herein, allelic variations of 7 genes were found through sequence alignment with the known genes, that is, TA7-RUF was allelic to PROG1, TA8-RUF was allelic to TIG1, SPR4-NIV was allelic to OsLG1, AN4-RUF was allelic to An-1, SH4-NIV was allelic to SH4, and both RC7-RUF and RC7-NIV were allelic to Rc. Meanwhile, 4 genes, TA11-NIV, SPR3-NIV, AN3-NIV, and AN4-NIV, were considered as the novel genes identified in these SSSLs, because of none known genes for the related domestication traits found in the chromosomal locations of them. The results indicated that the SSSLs would be precious germplasm resources for gene mining and utilization from wild rice species, and it laid the foundation for further analyses of the novel domestication genes to better understand the genetic basis in regulating the traits variation during domestication. The online version contains supplementary material available at 10.1186/s12284-023-00655-y.
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