Effects of Dietary Supplementation of Lactobacillus delbrueckii on Gut Microbiome and Intestinal Morphology in Weaned Piglets.
Effects of Dietary Supplementation of Lactobacillus delbrueckii on Gut Microbiome and Intestinal Morphology in Weaned Piglets.
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日粮补充德氏乳杆菌对断奶仔猪肠道微生物组和肠道形态的影响
DOI：
10.3389/fvets.2021.692389
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发表时间：
2021
影响因子：
3.2
通讯作者：
Huang XG
中科院分区：
文献类型：
--
作者：
Wang XL;Liu ZY;Li YH;Yang LY;Yin J;He JH;Hou DX;Liu YL;Huang XG
德尔布鲁氏乳杆菌是一种革兰氏阳性细菌，主要用于乳制品行业的酸奶和奶酪。本试验旨在研究在断奶仔猪饲粮中添加0.1%的德尔布吕氏乳杆菌对仔猪血清生化指标、肠道形态和生产性能的影响。选取80头初始体重为7.56±0.2 kg的健康断奶仔猪，随机分为2个饲喂组，每组4个重复（每个重复n = 10头）；分别饲喂基础饲粮（CON）和添加0.1%德布鲁克氏乳杆菌（LAC）的基础饲粮。结果表明：在试验第28天，饲粮中添加德尔布鲁氏乳杆菌改善了仔猪的生长性能，提高了血清HDL和胰岛素水平（p < 0.05）。肠道微生物分析显示，德尔布鲁氏乳杆菌显著降低拟杆菌门的相对丰度，但增加厚壁菌门的相对丰度。delbrueckii乳杆菌也显著提高了回肠细菌群落属水平上双歧杆菌和乳杆菌的相对丰度，但降低了未分类梭菌的相对丰度。此外，德尔布吕氏乳杆菌通过提高仔猪肠绒毛高度改善了仔猪黏膜形态（p < 0.05），显著提高了仔猪结肠食糜中丁酸、异丁酸和异戊酸的浓度，降低了试验第28天十二指肠和回肠的肠道pH值。综上所述，在断奶仔猪日粮中添加德尔布鲁氏乳杆菌可以改善肠道形态，调节肠道菌群，促进仔猪生长性能。
Lactobacillus delbrueckii is a Gram-positive bacterium mostly used in the dairy industry for yogurt and cheese. The present study was designed to evaluate the effects of Lactobacillus delbrueckii on serum biochemical parameters, intestinal morphology, and performance by supplementing at a dietary level of 0.1% in diets for weaned piglets. Eighty healthy weaned piglets (initial body weight: 7.56 ± 0.2 kg) were randomly divided into two feeding groups with four replicates in each group (n = 10 animals per replicate); piglets were fed with basal diet (CON) or basal diet containing 0.1% Lactobacillus delbrueckii (LAC). The results showed that dietary supplementation of Lactobacillus delbrueckii improved growth performance and increased serum HDL and insulin levels in piglets on the 28th day of the experimental time (p < 0.05). The gut microbe analysis revealed that Lactobacillus delbrueckii significantly decreased the relative abundance of the phyla Bacteroidetes, but increased the relative abundance of the phyla Firmicutes. The Lactobacillus delbrueckii also significantly increased the relative abundance of Bifidobacterium and Lactobacillus at the genus level of the bacterial community in the ileum, but decreased the relative abundance of unclassified Clostridiales. Moreover, Lactobacillus delbrueckii improved mucosal morphology by obtaining higher intestinal villus height (p < 0.05), significantly increasing the concentrations of butyrate, isobutyric acid, and isovaleric acid in colonic chyme of piglets, but decreasing the intestinal pH at the duodenum and ileum on the 28th day of the experimental time. In conclusion, dietary supplementation of Lactobacillus delbrueckii in the diet of weaned piglets can improve intestinal morphology and modulate the microbiota community to promote growth performance.
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影响因子：
3.3
作者：
He, Ting;Zhu, Yao-Hong;Wang, Jiu-Feng
通讯作者：
Wang, Jiu-Feng
DOI：
10.1016/j.aninu.2016.11.002
发表时间：
2017-03
期刊：
Animal nutrition (Zhongguo xu mu shou yi xue hui)
影响因子：
--
作者：
Dowarah R;Verma AK;Agarwal N
通讯作者：
Agarwal N
影响因子：
64.8
作者：
Fukuda, Shinji;Toh, Hidehiro;Ohno, Hiroshi
通讯作者：
Ohno, Hiroshi
DOI：
10.1023/a:1012213728917
发表时间：
2001-10-01
影响因子：
2.6
作者：
Chang, YH;Kim, JK;Park, YH
通讯作者：
Park, YH
影响因子：
4.4
作者：
Hutsko, S. L.;Meizlisch, K.;Lilburn, M. S.
通讯作者：
Lilburn, M. S.