Nutritional status and growth hormone regulate insulin-like growth factor binding protein (igfbp) transcripts in Mozambique tilapia.
Nutritional status and growth hormone regulate insulin-like growth factor binding protein (igfbp) transcripts in Mozambique tilapia.
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DOI：
10.1016/j.ygcen.2014.04.034
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发表时间：
2014-10-01
影响因子：
2.7
通讯作者：
Grau, E. Gordon
中科院分区：
文献类型：
--
作者：
Breves, Jason P.;Tipsmark, Christian K.;Stough, Beth A.;Seale, Andre P.;Flack, Brenda R.;Moorman, Benjamin P.;Lerner, Darren T.;Grau, E. Gordon
硬骨鱼的生长在很大程度上受生长激素（Gh）/胰岛素样生长因子（Igf）系统的活性控制。本研究从罗非鱼EST文库中初步鉴定了IGF结合蛋白（bp）1b、-2b、-4、-5a和-6b转录本。在莫桑比克罗非鱼（Oreochromis mossambicus）中，igfbps的组织表达谱显示igfbp 1b和-2b在肝脏中具有最高水平的表达，而igfbp 4、-5a和-6b在大多数其他组织中以相当的水平表达。我们比较了分解代谢条件下（禁食28天）肝脏igfbp 1b、igfbp 2b和igfbp 5a表达的变化，沿着Gh/Igf系统的关键成分，包括血浆Gh和Igf 1以及肝脏GH受体（ghr 2）、igf 1和igf 2表达。与血浆Gh升高和Igf 1水平降低平行，我们发现禁食动物肝脏igfbp 1b显著增加。然后我们测试了系统性Gh是否可以指导igfbps在肝脏中的表达。单次腹腔注射羊生长激素到垂体切除罗非鱼特异性刺激肝脏igfbp 2 b的表达沿着血浆Igf 1和肝脏ghr 2水平。我们的集体数据表明，肝脏内分泌信号在禁食可能涉及翻译后调节血浆Igf 1通过转向igfbp 1b的表达。因此，Igfbp 1b可以作为一个分子开关，以限制在罗非鱼Igf 1信号转导，此外，我们提供了新的细节异构体特异性调节igfbp的表达Gh。
Growth in teleosts is controlled in large part by the activities of the growth hormone (Gh)/insulin-like growth factor (Igf) system. In this study, we initially identified igf-binding protein (bp)1b, -2b, -4, -5a and -6b transcripts in a tilapia EST library. In Mozambique tilapia (Oreochromis mossambicus), tissue expression profiling of igfbps revealed that igfbp1b and -2b had the highest levels of expression in liver while igfbp4, -5a and -6b were expressed at comparable levels in most other tissues. We compared changes in hepatic igfbp1b, -2b and -5a expression during catabolic conditions (28 days of fasting) along with key components of the Gh/Igf system, including plasma Gh and Igf1 and hepatic gh receptor (ghr2), igf1 and igf2 expression. In parallel with elevated plasma Gh and decreased Igf1 levels, we found that hepatic igfbp1b increased substantially in fasted animals. We then tested whether systemic Gh could direct the expression of igfbps in liver. A single intraperitoneal injection of ovine Gh into hypophysectomized tilapia specifically stimulated liver igfbp2b expression along with plasma Igf1 and hepatic ghr2 levels. Our collective data suggest that hepatic endocrine signaling during fasting may involve post-translational regulation of plasma Igf1 via a shift towards the expression of igfbp1b. Thus, Igfbp1b may operate as a molecular switch to restrict Igf1 signaling in tilapia; furthermore, we provide new details regarding isoform-specific regulation of igfbp expression by Gh.
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影响因子：
4
作者：
Gabillard, Jean-Charles;Kamangar, Barzan Bahrami;Montserrat, Nuria
通讯作者：
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DOI：
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发表时间：
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影响因子：
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Grau, E. Gordon
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发表时间：
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影响因子：
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