Olfactory placode generates a diverse population of neurons expressing GnRH, somatostatin mRNA, neuropeptide Y, or calbindin in the chick forebrain.
Olfactory placode generates a diverse population of neurons expressing GnRH, somatostatin mRNA, neuropeptide Y, or calbindin in the chick forebrain.
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DOI：
10.1002/cne.25389
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发表时间：
2022-12
影响因子：
2.5
通讯作者：
Uchiyama, Yasuo
中科院分区：
文献类型：
--
作者：
Murakami, Shizuko;Ohki-Hamazaki, Hiroko;Uchiyama, Yasuo
关键词：
脊椎动物的嗅觉基板（OP）产生几种类型的迁移细胞，包括下丘脑促性腺激素释放激素（GnRH）产生神经元，这在生殖系统中起着重要作用。先前使用OP细胞标记的研究表明，OP来源的非GnRH细胞进入发育中的前脑；然而，它们的最终命运和表型尚不清楚。在鸡胚胎中，来自OP的迁移细胞亚群与GnRH神经元不同，会短暂表达生长抑素（SS）。我们假设这些细胞注定要发育成大脑神经元。在这项研究中，我们研究了发育过程中嗅觉前脑区SS mRNA的表达模式，以及OP来源的迁移细胞的目的地，包括表达SS mRNA的细胞。利用Tol2基因组整合系统诱导OP细胞的长期荧光蛋白表达，我们发现胚胎日(E) 3标记的OP来源的迁移细胞在E17-19存在于嗅觉神经和内侧前脑。保留在嗅觉神经中的绿色荧光蛋白（GFP）标记的GnRH神经元亚群被认为包括末梢神经神经元。在前脑中，GFP标记的细胞显示出与GnRH神经元相似的分布模式。大部分GFP标记的细胞表达成熟神经元标记物NeuN。在GFP标记的细胞中，GnRH神经元的比例很低，而其余的GnRH阴性神经元要么表达SS mRNA，神经肽Y，或calbindin D - 28k，要么不表达其中任何一种。这些结果表明，除了GnRH神经元外，还有多种OP来源的神经元细胞整合到鸡内侧前脑中。这项利用Tol2基因组整合系统进行的长期早期OP细胞标记研究表明，在孵化前前脑中OP来源的神经元并不局限于下丘脑GnRH神经元。它们表达生长抑素mRNA、神经肽Y、钙结合蛋白D - 28k，或者不表达这些物质。我们的研究结果表明，多种OP来源的神经元在鸡前脑中分化。
The olfactory placode (OP) of vertebrates generates several classes of migrating cells, including hypothalamic gonadotropin‐releasing hormone (GnRH)‐producing neurons, which play essential roles in the reproduction system. Previous studies using OP cell labeling have demonstrated that OP‐derived non‐GnRH cells enter the developing forebrain; however, their final fates and phenotypes are less well understood. In chick embryos, a subpopulation of migratory cells from the OP that is distinct from GnRH neurons transiently expresses somatostatin (SS). We postulated that these cells are destined to develop into brain neurons. In this study, we examined the expression pattern of SS mRNA in the olfactory‐forebrain region during development, as well as the destination of OP‐derived migratory cells, including SS mRNA‐expressing cells. Utilizing the Tol2 genomic integration system to induce long‐term fluorescent protein expression in OP cells, we found that OP‐derived migratory cells labeled at embryonic day (E) 3 resided in the olfactory nerve and medial forebrain at E17–19. A subpopulation of green fluorescent protein (GFP)‐labeled GnRH neurons that remained in the olfactory nerve was considered to comprise terminal nerve neurons. In the forebrain, GFP‐labeled cells showed a distribution pattern similar to that of GnRH neurons. A large proportion of GFP‐labeled cells expressed the mature neuronal marker NeuN. Among the GFP‐labeled cells, the percentage of GnRH neurons was low, while the remaining GnRH‐negative neurons either expressed SS mRNA, neuropeptide Y, or calbindin D‐28k or did not express any of them. These results indicate that a diverse population of OP‐derived neuronal cells, other than GnRH neurons, integrates into the chick medial forebrain. This long‐lasting early OP cell labeling study, utilizing the Tol2 genomic integration system, shows that OP‐derived neurons in the forebrain just before hatching are not specific to hypothalamic GnRH neurons. They express somatostatin mRNA, neuropeptide Y, calbindin D‐28k, or none of these substances. Our results indicate that diverse OP‐derived neurons are differentiated in the chick forebrain.
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DOI：
10.1073/pnas.94.4.1488
发表时间：
1997-02-18
影响因子：
11.1
作者：
Airaksinen, MS;Eilers, J;Meyer, M
通讯作者：
Meyer, M
影响因子：
3.3
作者：
Fornaro, M;Geuna, S;Giacobini-Robecchi, MG
通讯作者：
Giacobini-Robecchi, MG
GnRH、嗅觉缺失和促性腺激素低下性性腺功能减退症——我们在哪里？
影响因子：
7.4
作者：
Forni PE;Wray S
通讯作者：
Wray S
影响因子：
7.7
作者：
Aguillon, Raphael;Batut, Julie;Blader, Patrick
通讯作者：
Blader, Patrick
影响因子：
2.5
作者：
Mezey, Szilvia;Krivokuca, Dragan;Csillag, Andras
通讯作者：
Csillag, Andras