Advanced application of porcine intramuscular adipocytes for evaluating anti-adipogenic and anti-inflammatory activities of immunobiotics.
Advanced application of porcine intramuscular adipocytes for evaluating anti-adipogenic and anti-inflammatory activities of immunobiotics.
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DOI：
10.1371/journal.pone.0119644
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Kitazawa H
中科院分区：
文献类型：
--
作者：
Suzuki M;Tada A;Kanmani P;Watanabe H;Aso H;Suda Y;Nochi T;Miyazawa K;Yoda K;He F;Hosoda M;Saito T;Villena J;Kitazawa H
我们之前建立了克隆猪肌内前脂肪细胞（PIP）系，并建立了从PIP细胞获得功能性成熟脂肪细胞的方案。我们假设PIP细胞和成熟脂肪细胞都可能是有用的体外工具，可以增加我们对脂肪组织免疫生物学的理解，以及选择和研究能够调节脂肪细胞免疫反应的免疫调节益生菌（免疫益生菌）。在这项研究中，我们研究了PIP细胞和成熟脂肪细胞对TNF-α刺激的免疫生物学反应。此外，我们评估了免疫微生物通过Peyer’s patches （PPs）免疫能细胞改变猪脂肪组织脂肪形成和免疫功能的可能性。用不同的益生菌菌株处理猪PPs免疫细胞；我们评估了益生菌刺激免疫细胞条件培养基对PIP细胞和成熟脂肪细胞的影响。乳酸菌GG和乳酸菌TMC0356对TNF-α攻毒后脂肪细胞的促炎因子和负性调节因子（A20、Bcl-3和mbp -1）的表达均有显著降低。本研究结果表明，TNF-α-挑战脂肪细胞中IL-6和MCP-1的产生以及A20和Bcl-3的下调可作为筛选潜在免疫生物菌株的生物标志物。考虑到几项体内和体外研究清楚地证明了乳酸杆菌GG和乳杆菌TMC0356对脂肪炎症的有益作用，本工作的结果表明，PIP细胞和猪脂肪细胞可用于筛选和选择新的免疫生物菌株，这些菌株在口服时可能具有功能调节脂肪炎症的潜力。
We previously established a clonal porcine intramuscular preadipocyte (PIP) line and we were able to establish a protocol to obtain functional mature adipocytes from PIP cells. We hypothesized that both PIP cells and mature adipocytes are likely to be useful in vitro tools for increasing our understanding of immunobiology of adipose tissue, and for the selection and study of immunoregulatory probiotics (immunobiotics) able to modulate adipocytes immune responses. In this study, we investigated the immunobiology of PIP cells and mature adipocytes in relation to their response to TNF-α stimulation. In addition, we evaluated the possibility that immunobiotic microorganisms modify adipogenesis and immune functions of porcine adipose tissue through Peyer’s patches (PPs) immune-competent cells. We treated the porcine PPs immune cells with different probiotic strains; and we evaluated the effect of conditioned media from probiotic-stimulated immune cells in PIP cells and mature adipocytes. The Lactobacillus GG and L. gasseri TMC0356 showed remarkable effects, and were able to significantly reduce the expression of pro-inflammatory factors and negative regulators (A20, Bcl-3, and MKP-1) in adipocytes challenged with TNF-α. The results of this study demonstrated that the evaluation of IL-6, and MCP-1 production, and A20 and Bcl-3 down-regulation in TNF-α-challenged adipocytes could function as biomarkers to screen and select potential immunobiotic strains. Taking into consideration that several in vivo and in vitro studies clearly demonstrated the beneficial effects of Lactobacillus GG and L. gasseri TMC0356 in adipose inflammation, the results presented in this work indicate that the PIP cells and porcine adipocytes could be used for the screening and the selection of new immunobiotic strains with the potential to functionally modulate adipose inflammation when orally administered.
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影响因子：
3.8
作者：
Kim S;Kim JH;Park BO;Kwak YS
通讯作者：
Kwak YS
DOI：
10.1152/ajpendo.00110.2003
发表时间：
2003-09-01
影响因子：
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作者：
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通讯作者：
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DOI：
10.1016/j.bbrc.2012.12.121
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影响因子：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
HOTAMISLIGIL, GS;SHARGILL, NS;SPIEGELMAN, BM
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